铅胁迫下柳树4个无性系生长和生理特性的变化  

Growth and physiological traits of four Salix clones under lead stress

作　　者：黄瑞芳[1,2] 张忠 曹瑶 教忠意 施士争[1,2] Huang Ruifang;Zhang Zhong;Cao Yao;Jiao Zhongyi;Shi Shizheng(Jiangsu Academy of Forestry, Nanjing 211153, China;The Jiangsu Provincial Infrastructure for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014, China;Productivity Center of Shuyang County, Shuyang 223600, China)

机构地区：[1]江苏省林业科学研究院,江苏南京211153 [2]江苏省农业种质资源保护与利用平台,江苏南京210014 [3]沭阳县生产力促进中心,江苏沭阳223600

出　　处：《江苏林业科技》2020年第2期35-39,共5页Journal of Jiangsu Forestry Science & Technology

基　　金：江苏省农业科技自主创新资金项目“灌木柳树修复铅污染土壤的专用品种选育”;江苏省林业科技创新与推广项目“抗逆速生灌木柳新品种区域性试验与示范”(LYKJ[2019]43)。

摘　　要：该研究以4个灌木柳树无性系为试验材料,采用水培法进行铅胁迫处理[0,20,40,80 mg/L Pb(NO3)2],测定其生物量及部分生理指标的变化,以明确不同无性系用于铅修复的潜力。结果显示:4个灌木柳树的变化整体趋势一致。随着铅质量浓度的升高,各柳树无性系的生物量呈下降趋势,SOD酶活性呈增加趋势,POD酶活性呈先增加后降低的趋势,MDA含量呈增加趋势,可溶性蛋白含量呈降低趋势。采用主成分分析法和加权隶属函数法进行分析,得出4个无性系耐铅能力强弱的顺序为P336>P63>P1024>P61。Four shrub willow clones by hydroponics were selected to study the physiological change under different Pb stress[0,20,40 and 80 mg/L Pb(NO3)2].The change in biomass and other physiological indexes were used to determine the potential of different clones for Pb restoration.The results showed almost consistent in the rule of physiological change overall trend of the four clones.With the increase of Pb concentration,the biomass of all willow clones decreased,SOD enzyme activity increased,POD enzyme activity increased first and then decreased,MDA content increased,and soluble protein content decreased.By principal component analysis and weighted membership function analysis,it was concluded that the capacity of Pb endurance of the four clones was ranked as P336>P63>P1024>P61.

关 键 词：铅 胁迫 柳树 无性系 生理特性 

分 类 号：Q945.78[生物学—植物学] S792.12[农业科学—林木遗传育种]