Effects of cluster needling of scalp acupuncture on neurofilament protein 200 and signal transducer and activator of transcription 3 in rats with acute cerebral ischemia  

作　　者：Xue Fang Jinxia Ni Buyi Su Huiyan An Miaomiao Li Jie Wang Xiaona Wu 

机构地区：[1]Department of Acupuncture and Moxibustion,Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan,250011,China [2]Department of Acupuncture and Moxibustion,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing,100700,China [3]Rehabilitation Center,Shenyang Jishuitan Hospital,Shenyang,110000,China

出　　处：《Journal of Traditional Chinese Medical Sciences》2020年第1期82-86,共5页中医科学杂志（英文）

基　　金：This study was supported by grants from the National Natural Science Foundation of China(grant No.81473775).

摘　　要：Objective:Cluster needling at scalp acupoints has showed satisfying effects with acute cerebral ischemia in clinic whereas the mechanisms are not yet clear completely.This study investigated the influence of cluster needling at scalp acupoints on neurological function,as well as on neurofilament protein 200(NF200)and signal transducer and activator of transcription 3(STAT3)expression,in rats with acute cerebral ischemia.Methods:Fifty-four Sprague-Dawley rats were randomly assigned in equal numbers to the false operation(group F),model(group M),or cluster needling scalp acupuncture(group C)groups.Each group was divided into three subgroups,of six rats each,by acupuncture treatment time(24 h,7 days,and 14 days).The rat local cerebral ischemia model was prepared using a modified suture occlusion method.Group C rats were treated by cluster needling scalp acupuncture,while groups F and M did not receive acupuncture treatment.Neurological effects were evaluated using the Longa score.NF200 and STAT3 expression were measured by western blotting.Results:At 24 h,there were no statistical difference between group C and group M in nerve function(P>.05).On days 7 and 14,nerve function scores in group C were significantly lower than that in group M(respectively were P<.05 and P<.01).In addition,on days 14,expression of NF200 was significantly higher in group C compared with group M(P<.05).Compared with group M,STAT3 expression was also higher in group C on days 7 and 14,although these differences were not statistically significant(both P>.05).Conclusion:Cluster needling scalp acupuncture were efficient in improving nerve function scores in rats with cerebral ischemia,and promoting the recovery of motor function.These improvements were associated with increases in NF200 and STAT3 expression.

关 键 词：Cluster needling scalp acupuncture Acute cerebral ischemia NF200 STAT3 Suture occlusion method Rat model 

分 类 号：R245[医药卫生—针灸推拿学]