Xijiao Dihuang Decoction combined with Yinqiao Powder reverses influenza virus-induced F-actin reorganization in PMVECs by inhibiting ERM phosphorylation  

作　　者：Zinan Xuan Ying Wu Chenyue Zhang Shujing Zhang Xiangyang Chen Shuyu Li Yu Hao Qian Wang Xudan Wang Shu Zhang 

机构地区：[1]Department of Microbiology and Immunology,Beijing University of Chinese Medicine,Beijing 100029,PR China [2]Department of Integrative Oncology,Fudan University Shanghai Cancer Center,Shanghai 200032,PR China

出　　处：《Journal of Traditional Chinese Medical Sciences》2016年第1期50-58,共9页中医科学杂志（英文）

基　　金：This work was supported by funding from the National Natural Science Foundation of China(Grant Nos.81473520 and 81102697).

摘　　要：Objective:It has been documented that ezrin/radixin/moesin(ERM)phosphorylation by the p38 mitogen-activated protein kinase(MAPK),Rho/ROCK,and protein kinase C(PKC)pathways leads to filamentous actin(F-actin)reorganization and microvascular endothelial cell hyperpermeability.In this study,we investigated the effects of Xijiao Dihuang Decoction combined with Yinqiao Powder(XDY)on influenza virus(IV)-induced F-actin restructuring and ERM phosphorylation regulated by the Rho/Rho kinase 1(ROCK),p38 MAPK,and PKC signaling pathways in pulmonary microvascular endothelial cells(PMVECs).Methods:Serum containing XDY(XDY-CS;13.8 g/kg)was acquired using standard protocols for serum pharmacology.Primary PMVECs were obtained from male Wistar rats and cultured.After adsorption of IV A(multiplicity of infection,0.01)for 1 h,medium with 20%XDY-CS was added to the PMVECs.The distributions of F-actin and phosphorylated ERM were determined by confocal microscopy,and F-actin expression was measured by flow cytometry.The expression levels of ROCK1,phosphorylated myosin phosphatase target-subunit(p-MYPT),phosphorylated MAPK kinase,phosphorylated p38(p-p38),phosphorylated PKC(p-PKC),and phosphorylated ERM(p-ERM)were determined by western blotting.Results:F-actin reorganization in IV-infected PMVECs was reversed by XDY-CS treatment,which was accompanied by reduced p-ERM production.The p-ERM protein accumulated at plasma membrane of PMVECs infected with IV,which was also inhibited by XDY-CS treatment.

关 键 词：Xijiao Dihuang Decoction combined with Yinqiao Powder Influenza virus Pulmonary microvascular endothelial cells Filamentous actin Ezrin/radixin/moesin 

分 类 号：R285[医药卫生—中药学]