Biotransformation of 6-deoxypseudoanisatin by Synechocystis sp. PCC6803  

作　　者：Zhi Wang Xiaodong Cui Chunmei Wang Jianmei Huang Di Geng 

机构地区：[1]School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100102,China

出　　处：《Journal of Traditional Chinese Medical Sciences》2014年第2期135-139,共5页中医科学杂志（英文）

基　　金：Beijing University of Chinese Medicine(2013-JYBZZ-JS-139)Independent Project Topics Foundation.

摘　　要：Objective:To explore the ability of Synechocystis sp.PCC6803 in transforming 6-deoxypseudoanisatin.Methods:The experiment was performed by incubating 6-deoxypseudoanisatin with the freshwater cyanobacterium Synechocystis sp.PCC6803 under continuous white light at 30C for 5 days.The crude converted product was detected using thin-layer chromatography(TLC)and further analyzed using high-performance liquid chromatography(HPLC)as well as HPLC with electron spray ionization mass spectrometry(HPLC-ESI-MS).Results:TLC results showed that 6-deoxypseudoanisatin was converted into a less polar product.HPLC and MS data indicated that the retention time of the converted product increased in comparison with the standard of 6-deoxypseudoanisatin.Conclusion:Thus,the study appears to demonstrate that Synechocystis sp.PCC6803 can transform 6-deoxypseudoanisatin.The polarity of the converted product is less than that of 6-deoxypseudoanisatin.

关 键 词：Synechocystis sp.PCC6803 6-deoxypseudoanisatin Seco-prezizaane-type sesquiterpene lactone BIOTRANSFORMATION CYANOBACTERIUM 

分 类 号：R284.1[医药卫生—中药学]