Anti-cancer activity of Tonglian decoction against esophageal cancer cell proliferation through regulation of the cell cycle and PI3K/Akt signaling pathway  

作　　者：Yongsen Jia Lijuan Qin Chunhua Jiang Qing Lin Fuling Tian Huijuan Cao Xin Yan 

机构地区：[1]College of Chinese Medicine,North China University of Science and Technology,Tangshan 063000,China [2]School of Basic Medical Science,North China University of Science and Technology,Tangshan 063000,China

出　　处：《Journal of Traditional Chinese Medical Sciences》2015年第2期120-126,共7页中医科学杂志（英文）

基　　金：the National Natural Science Fund of China(81101912);Natural Science Fund of Hebei Province of China(H2013209053);Scientific and Technological Project of Administration of Traditional Chinese Medicine of Hebei(2014185)。

摘　　要：Objective:The purpose of this study was to observe the anti-cancer activity of Tonglian decoction(TD)on esophageal cancer(EC)cells in vitro,and to elucidate the related molecular mechanisms in the cell cycle and PI3K/Akt signaling pathway.Methods:EC9706 cells were cultured in RPMI 1640 medium supplemented with 10%calf serum at 37
C in a 5%CO2 incubator.The cells were treated with rat serum containing TD or the serum of rats administered Xiaoaiping as a positive control drug.Cell proliferation was assessed by methylthiazolyldiphenyl-tetrazolium bromide assays.Cell morphology was observed under a microscope.The cell cycle was examined by flow cytometry.Protein expression in the PI3K/Akt signaling pathway was measured by western blotting.Results:TD mainly inhibited cell proliferation.Concentrations of 50%cell inhibition by rat serum containing TD or Xiaoaiping were 73.6 and 153.8 mL/mL,respectively.TD also influenced cell morphology characterized by small shrunken cells.Cell colonies became small and the cell proliferation rate was slower.In cell cycle analysis,the percentage of cells in S phase was decreased significantly by TD and Xiaoaiping compared with the blank control group(P<.05).Western blotting showed that serum containing TD strongly downregulated EGFR,PI3K,Akt,p-Akt,and mTOR expression compared with the blank control group(P<.05).Conclusion:TD could inhibit EC9706 carcinoma cell proliferation by blocking the cell cycle progression in S phase.The possible mechanism was inhibition of multiple targets in the PI3K/Akt signaling pathway by TD.

关 键 词：Tonglian decoction Esophageal cancer EC9706 cells Cell cycle PI3K/AKT 

分 类 号：R285.5[医药卫生—中药学]