金纳米电极对多巴胺和5-羟色胺的电化学富集和拉曼光谱检测  

Electrochemical Enrichment and Raman Spectroscopy Detection of Dopamine and 5-Hydroxytryptamine by Gold Nanoelectrode

作　　者：杨丹 周晶 李语静 赵锐 冯文南 何劲 王哲[3] 杨国程[2] YANG Dan;ZHOU Jing;LI Yu-Jing;ZHAO Rui;FENG Wen-Nan;HE Jin;WANG Zhe;YANG Guo-Cheng(College of Material Science and Engineering,Changchun University of Technology,Changchun 130012,China;College of Chemistry and Life Science,Changchun University of Technology,Changchun 130012,China;College of Chemical Engineering,Changchun University of Technology,Changchun 130012,China)

机构地区：[1]长春工业大学材料科学与工程学院,长春130012 [2]长春工业大学化学与生命科学学院,长春130012 [3]长春工业大学化学工程学院,长春130012

出　　处：《分析化学》2020年第6期742-749,共8页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21673023,21773017)资助。

摘　　要：对小分子神经递质多巴胺(Dopamine,DA)和5-羟色胺(5-Hydroxytryptamine,5-HT)的同时分析和检测是生命科学领域中的研究热点。单一的电化学检测手段难以有效地识别分析物,因此,多模量的同时分析尤为重要。本研究在碳纳米电极(Carbon-nanoelectrode nanopipette,CNE-NP)的基础上,通过电化学反应在其尖端沉积金,构建了一种具有电化学特性和拉曼活性的双功能金纳米电极(Gold-nanoelectrode nanopipette,GNE-NP)。经过交流介电泳(Dielectrophoresis,DEP)富集后,可用于对低浓度的DA和5-HT的检测分析。研究结果表明,此电极实现了两者的电化学响应的增强和区分。此外,引入的银纳米颗粒(AgNPs)作为拉曼增强因子和捕获分析物的纳米颗粒,同样地经过DEP富集方法,使分析物的拉曼信号增强了2个数量级,且能对共混物进行特征峰的区分。将此电极应用于血浆样品分析,可得到富集增强的电化学响应,但电化学响应会受到富集次数的限制。Simultaneous detection of small molecule neurotransmitters dopamine(DA)and 5-hydroxytryptamine(5-HT)has become a research hotpot in the field of life sciences.A single electrochemical detection mean is difficult to accurately identify analytes.Therefore,it is particularly important for the multimode simultaneous detection and analysis.In this study,a dual-functional gold nanoelectrode-nanopipette based on the nanopipette was constructed with electrochemical properties and Raman activity.On the basis of the carbon nanoelectrode-nanopipette,the apex of it deposited gold by electrochemistry and then enriched analytes by dielectrophoresis(DEP)method,which could be used for the detection of DA and 5-HT in low concentration.The results showed that this nanoelectrode achieved the enhanced electrochemical responses and differentiated the two.The introduced silver nanoparticles(AgNPs)were used as Raman enhancement factor and as the nanoparticles of analytes capturer.By utilizing DEP enrichment method,the Raman signal was enhanced by two orders of magnitude and the characteristic peaks of DA and 5-HT in the mixture were identified.When this nanoelectrode was applied in the analysis of serum samples,as the number of enrichment reached up to the third time,the enhanced electrochemical response could also be obtained,but this response was limited by the number of enrichments.

关 键 词：基于纳米孔道的金纳米电极 多巴胺 5-羟色胺 介电泳富集 表面增强拉曼光谱 

分 类 号：O657[理学—分析化学] R446.1[理学—化学]