基于石墨烯增敏的甲基对硫磷分子印迹电化学传感器的研制  

Electrochemical Sensor Based on Molecularly Imprinted Polymer and Graphene Modified Electrode for Determination of Methyl Parathion

作　　者：吴佳雯 谢周鉴 谭学才 胡琪 雷福厚 WU Jia-Wen;XIE Zhou-Jian;TAN Xue-Cai;HU Qi;LEI Fu-Hou(School of Chemistry and Chemical and Engineering,Guangxi University for Nationalities,Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry,Guangxi Key Laboratory of Chemistry and Engineering of Forest Products,Guangxi Zhuang Autonomous Region Institute of Product Quality Inspection,Nanning 530006,China)

机构地区：[1]广西民族大学化学化工学院,广西高校食品安全与药物分析化学重点实验室,广西林产化学与工程重点实验室,广西壮族自治区产品质量检验研究院,南宁530006

出　　处：《分析化学》2020年第6期757-764,共8页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21365004);广西重点研发计划项目(No.桂科AB18126048);广西科技重大专项项目(No.桂科AA18118013-10);广西科技基地和人才专项项目(No.AD18126005);广西高校中青年教师基础能力提升项目(No.2017KY0164);广西民族大学校级科研青年基金项目(No.2018MDQN004)资助。

摘　　要：以甲基对硫磷为模板分子,甲基丙烯酸为功能单体,马来松香丙烯酸乙二醇酯为交联剂,在石墨烯修饰的玻碳电极表面合成分子印迹聚合物膜,制备分子印迹电化学传感器,用于检测有机磷农药甲基对硫磷。采用循环伏安法、微分脉冲伏安法、电化学交流阻抗法研究传感器的电化学特性及分析特性。结果表明,在最佳检测条件下,传感器的峰电流与甲基对硫磷浓度在1.0×10^-7~8.0×10^-5 mol/L范围内呈线性关系,线性方程为Ip(μA)=-0.65199-0.02387C(μmol/L),相关系数R2为0.9922,检出限为5.0×10^-8 mol/L(S/N=3)。构建了传感器的动力学吸附模型,测得印迹传感器的结合速率常数k=20.2758s。将此传感器用于番茄样品中甲基对硫磷的检测,加标回收率为95.0%~100.3%。A molecularly imprinted electrochemical sensor based on graphene modified electrode was fabricated for determination of methyl parathion(MP). The molecularly imprinted polymer membrane was synthesized on the surface of graphene-modified glassy carbon electrode by using MP as template, methacrylic acid as functional monomer and ethylene glycol maleic rosinate acrylate as cross linker. The electrochemical properties of the sensor were investigated by cyclic voltammetry(CV), differential pulse voltammetry(DPV) and electrochemical impedance spectroscopy(EIS). Under optimal conditions, the peak current of the sensor showed a good linear correlation with the MP concentration in the range of 1.0×10^-7-8.0×10^-5 mol/L, with a detection limit of 5.0×10^-8 mol/L(S/N=3). The linear regression equation was Ip(μA)=-0.65199-0.02387C(μmol/L), with a correlation coefficient(R2) of 0.9922. The adsorption model of the imprinted sensor was investigated, and the binding rate constant k was 20.2758 s. The sensor was successfully applied to determination of MP in tomato samples with recoveries of 95.0% to 100.3%.

关 键 词：甲基对硫磷 石墨烯 分子印迹电化学传感器 马来松香丙烯酸乙二醇酯 

分 类 号：O657.1[理学—分析化学] X85[理学—化学]