化学衍生-液相色谱-串联质谱法测定加热卷烟烟气中的醛酮类香味成分  

Determination of Aldehyde and Ketone Flavor Compounds in Mainstream Smoke of Heated Tobacco Products by Chemical Derivatization-Liquid Chromatography-Tandem Mass Spectrometry

作　　者：罗彦波[1] 陈小静[1] 李翔宇[1] 张洪非[1] 朱风鹏[1] 姜兴益[1] 庞永强[1] LUO Yan-Bo;CHEN Xiao-Jing;LI Xiang-Yu;ZHANG Hong-Fei;ZHU Feng-Peng;JIANG Xing-Yi;PANG Yong-Qiang(China National Tobacco Quality Supervision and Test Center,Zhengzhou 450001,China)

机构地区：[1]国家烟草质量监督检验中心,郑州450001

出　　处：《分析化学》2020年第6期794-801,共8页Chinese Journal of Analytical Chemistry

基　　金：国家烟草质量监督检验中心科技创新项目(No.512018CA0050)资助。

摘　　要：建立了化学衍生结合液相色谱-串联质谱(LC-MS/MS)检测加热卷烟烟气中9种醛酮类香味成分的分析方法。样品和标准溶液中的目标分析物分别用1-(2-肼基-2-氧代乙基)吡啶-1-溴化物(吉拉德P试剂,GP)和d5-GP衍生,以标准溶液的衍生化产物作为稳定同位素内标,采用多反应监测模式检测。在最优的衍生化反应条件下, 9种醛酮类香味成分在各自浓度范围内线性关系良好,标准曲线相关系数(R2)≥0.9937,检出限为0.07~12.66 ng/mL,相对标准偏差为0.2%~11.6%。9种醛酮类香味成分在3个加标水平下的回收率为82.5%~118.0%。本方法可用于加热卷烟烟气中醛酮类香味成分的检测。A method for detection of 9 kinds of aldehyde and ketone flavor compounds in mainstream smoke of heated tobacco products based on liquid chromatography-tandem mass spectrometry(LC-MS/MS) was established. The target analytes in samples and standard solution were derivatized with 1-(2-hydrazinyl-2-oxoethyl)pyridin-1-bromide(Girard reagent P, GP) and d5-GP, respectively. The derivative products of the standard solution were used as stable isotope internal standards, and the target analytes were analyzed by multiple reaction monitoring mode. The limits of detection were 0.07-12.7 ng/mL under the optimum derivatization conditions. The target analytes exhibited a good linearity with the standard curve correlation coefficients(R2) of not less than 0.9937, and the relative standard deviation was 0.2%-11.6%. The recoveries of real samples at three spiked levels were 82.5%-118.0%. Finally, the method was successfully applied to the analysis of aldehyde and ketone flavor compounds in mainstream smoke of heated tobacco products.

关 键 词：化学衍生 液相色谱-串联质谱 加热卷烟 醛酮类香味成分 

分 类 号：O657.63[理学—分析化学] TS411[理学—化学]