TGF-β1+915G/C基因多态性与慢性肾炎临床表现的相关性分析  

Analysis on the correlation of TGFβ1+915G/C polymorphism with clinical manifestations of chronic glomerulonephritis

作　　者：项新[1] 冯振伟[1] 黄典胜 李洁[1] 何坤[1] 陆玲娜[1] XIANG Xin;FENG Zhenwei;HUANG Diansheng;LI Jie;HE Kun;LU Lingna(National Hospital Affiliated to Guangxi Medical University,Nanning 530001,Guangxi,China)

机构地区：[1]广西医科大学附属民族医院,广西南宁530001

出　　处：《现代中西医结合杂志》2020年第17期1843-1846,共4页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：崇左市研究与技术开发计划项目(崇科攻13042303)。

摘　　要：目的探讨转化生长因子β1(TGF-β1)+915G/C基因多态性与慢性肾炎临床表现的相关性,从基因水平探讨慢性肾炎的发病机制。方法选择2013年1月—2018年12月在广西医科大学附属民族医院肾内科住院的慢性肾炎患者60例作为慢性肾炎组,同期健康体检肝肾功能和血尿常规正常、无原发或继发性肾小球疾病及肿瘤者40例作为对照组,利用聚合酶链反应-限制性片段长度多态性分析法鉴定2组TGF-β1+915G/C基因型,收集慢性肾炎组患者临床主要指标数据[血压、尿常规、24 h尿蛋白定量及血清C反应蛋白(CRP)、SCr、尿素氮(BUN)、白蛋白(ALB)、总胆固醇(TC)、三酰甘油(TG)、TGF-β1水平],比较慢性肾炎组不同基因型患者临床指标的差异性。结果2组均检出CC型、GC型、GG型3种基因型,2组TGF-β1+915G/C基因型及等位基因频率分布情况比较差异均无统计学意义(P均>0.05)。GG基因型患者的24 h蛋白尿量、血清TGF-β1水平和高血压发生率均明显高于GC+CC基因型患者(P均<0.05),而血TG、TC、ALB、BUN、SCr、CRP水平和血尿发生率与GC+CC基因型患者比较差异均无统计学意义(P均>0.05)。结论TGF-β1+915G/C基因多态性能影响血清TGF-β1水平,可能与慢性肾炎较重的蛋白尿及高血压发生相关,但与慢性肾炎的发病易感性不相关。Objective It is to investigate the clinical correlation of TGF-β1+915G/C polymorphism with chronic glomerulonephritis,thus to discuss the pathogenesis of chronic nephritis from the genetic level.Methods Sixty patients with chronic nephritis who were hospitalized in the Department of Nephrology of the Nationalities Hospital Affiliated to Guangxi Medical University from January 2013 to December 2018 were selected as the chronic nephritis group.During the same period,40 patients with normal liver and kidney function and normal hematuria,with no primary or secondary glomerular disease and tumor were used as control group.Polymerase chain reaction-restriction fragment length polymorphism analysis was used to identify TGF-β1+915G/C genotype of the two groups,and the main clinical data[blood pressure,urine routine,24 h urine protein quantification and serum C-reactive protein(CRP),SCr,urea nitrogen(BUN),albumin(ALB),total cholesterol(TC),triacylglycerol(TG),TGF-β1]of the patients with chronic nephritis were collected to compare the differences in clinical indicators of patients with different genotypes in the chronic nephritis group.Results There were three types of genotypes CC,GC and GG detected in the two groups.There was no statistically significant difference in the TGF-β1+915G/C genotype and allele frequency distribution between the two groups(all P>0.05).The 24 h proteinuria,serum TGF-β1 level and the incidence of hypertension in patients with GG genotype were significantly higher than those patients with GC+CC genotype(P<0.05),while the levels of blood TG,TC,ALB,BUN,SCr,CRP and incidence of hematuria were not statistically different from those of GC+CC genotypes(all P>0.05).Conclusion TGF-β1+915G/C gene polymorphism could influence serum level of TGF-β1,the mechanism may be related to the occurrence of hepatic proteinuria and hypertension in chronic nephritis,but not to the susceptibility of chronic nephritis.

关 键 词：转化生长因子Β1 慢性肾炎 基因多态性 相关性 

分 类 号：R692.3[医药卫生—泌尿科学]