申克孢子丝菌4Hppd基因结构功能的生物信息学分析  

Bioinformatics analysis of 4Hppd gene in Sporothrix schenckii

作　　者：吴晓雁 张静[2] 黄家敏 孙瑶 黄晓雯 高文超[1] 吴榕 陈三妹 黄怀球[1] WU Xiao-yan;ZHANG Jing;HUANG Jia-min;SUN Yao;HUANG Xiao-wen;GAO Wen-chao;WU Rong;CHEN San-mei;HUANG Huai-qiu(Department of Dermatology,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China;Department of Dermatology,Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120,China;Department of Dermatology,Shenzhen People s Hospital,Shenzhen 518020,China;Department of Dermato-Venereolgy,The First Affiliated Hospital of Anhui Medical University,Hefei 230022,China;Department of Dermatology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;Department of General Medicine,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630)

机构地区：[1]中山大学附属第三医院皮肤科,广东广州510630 [2]中山大学孙逸仙纪念医院皮肤科,广东广州510120 [3]深圳市人民医院皮肤科,广东深圳518020 [4]安徽医科大学第一附属医院皮肤性病科,安徽合肥230022 [5]南方医科大学南方医院皮肤科,广东广州510515 [6]中山大学附属第三医院全科医学,广东广州510630

出　　处：《皮肤性病诊疗学杂志》2020年第2期79-82,88,共5页Journal of Diagnosis and Therapy on Dermato-venereology

基　　金：国家自然科学基金(编号:81974300,81371746);中山大学三大建设超算专项(编号:82000-31143405)。

摘　　要：目的:初步预测及分析申克孢子丝菌(Sporothrix schenckii,Ss.)对羟苯基丙酮酸双加氧酶基因(4Hppd)编码蛋白的结构和生物学功能特征。方法:应用生物信息学在线工具预测分析Ss.4Hppd编码蛋白的理化性质及功能特征。结果:Ss.4Hppd基因编码蛋白含对羟苯基丙酮酸双加氧酶保守域,由477个氨基酸构成,理论分子量为52.0 kD,等电点为5.98,半衰期较长,该蛋白为非分泌蛋白,定位于线粒体中。其二级结构以无规则卷曲及α-螺旋为主,另外还存在乙二醛酶功能域及丰富的磷酸化位点,与其他致病真菌同源蛋白有较高的相似度。结论:预测Ss.4HppD位于线粒体中,具有乙二醛酶功能域结构功能特点及丰富修饰位点,可为其表达、晶体结构分析及小分子抑制剂开发提供参考。Objective:To predict biological characterisics of 4-HppD,encoded by 4Hppd in Sporothrix schenckii.Methods:Mutiple bioinformatics online tools were used to analyse the sequence.Results:Ss.4HppD,located in mitochodria,was formed by 477 amino acids and its theoretical molecular weight is 52.0 kD.The isoelectric point was about 5.98,with a long half life.The dominant secondary structures in Ss.4HppD were random coil and alpha helix.Besides,Ss.4HppD had a similar tertiary structure with its homologue proteins,a glyoxalase motif at the carboxy end and multiple phosphorylation sites.Conclusion:Ss.4HppD was predicted to located in mitochondria,with a glyoxalase motif and various modification sites,which would facilitate the further researches in the protein including protein expression,function mechanism and inhibitior development.

关 键 词：申克孢子丝菌 对羟苯基丙酮酸双加氧酶基因 生物信息学分析 

分 类 号：R379[医药卫生—病原生物学]