20株临床来源耐药粪肠球菌的21种耐药元件基因检测与分析  

Detection and analysis of 21 drug resistance element genes of 20 drug resistant Enterococcus faecalis from clinical sources

作　　者：丘江 张洁 糜祖煌[2] QIU Jiang;ZHANG Jie;MI Zuhuang(Departement of Clinical Laboratory,Hubei Jianghan Oilfield General Hospital,Qianjiang 433124;Wuxi Clone Gene-tech Institute,Wuxi 214026,China)

机构地区：[1]湖北江汉油田总医院检验科,湖北潜江433121 [2]无锡市克隆遗传技术研究所,江苏无锡214000

出　　处：《南京医科大学学报（自然科学版）》2020年第4期528-532,共5页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:调查20株耐药粪肠球菌株可能存在的耐药基因状况和菌株间的亲缘关系。方法:收集2015年1月-2018年12月湖北省江汉油田总医院住院患者分离的20株耐药粪肠球菌株,用PCR法检测1种青霉素耐药基因、4种氨基糖苷类耐药基因、6种大环内酯类耐药基因、1种链阳菌素耐药基因、2种四环素耐药基因、3种万古霉素耐药基因、2种季胺类消毒剂耐药基因、1种可移动遗传元件标志基因,最后对检测结果作样本聚类分析(UPGMA法)。结果:20株耐药粪肠球菌分别检出1~7种耐药元件基因,包括1种青霉素耐药基因、3种氨基糖苷类耐药基因、2种大环内酯类耐药基因、1种四环素耐药基因、2种万古霉素耐药基因、1种可移动遗传元件标志基因。菌株亲缘关系分析可见20株耐药粪肠球菌可分为A、B 2群。B群又可分为B-1、B-2亚群。B-1亚群有2个克隆播散(4号株-14号株-17号株、8号株与11号株),B-2亚群有1个克隆播散(9号株与12号株)。结论:青霉素耐药基因、氨基糖苷类耐药基因、大环内酯类耐药基因、四环素耐药基因、万古霉素耐药基因和可移动遗传元件是导致本组粪肠球菌耐药的重要原因。Objective:To investigate the distribution of resistant determinants in drug-resistant Enterococcus faecalis(E. faecalis),and their relationships among strains. Methods:Twenty strains of E. faecalis were collected from Jianghan Oil Field General Hospital in Hubei from January 2015 to December 2018. Then,1 kind of resistance gene to penicillin, 4 kinds of resistance genes to aminoglycosides,6 kinds of resistance genes to macrolides,1 kind of resistance gene to streptogramin,2 kinds of resistance genes to tetracycline,3 kinds of resistance genes to vancomycin,2 kinds of resistance genes to quaternary ammonium disinfectants,2 kinds of genetic markers of mobile genetic elements were analyzed by PCR. At last,sample cluster analysis was performed(UPGMA method).Results:At least 1 resistant determinants,and 7 at the most,were positive in 20 strains of drug-resistant E. faecalis. And 1 kind of resistance gene to penicillin,3 kinds of resistance genes to aminoglycosides,1 kind of resistance genes to macrolides,1 kind of resistance genes to tetracycline,2 kinds of resistance genes to vancomycin,1 kind of genetic markers of mobile genetic elements were positive. The analysis of phylogenetic relationship showed that 20 strains could be divided into A and B two groups. The B group can be divided into 2 subgroups:B-1 and B-2. There were 2 clones in the subgroup B-1(strain No. 4-14-17,and strain No. 8-11). As well as a clone in the subgroup B-2(strain No. 9-12). Conclusion:Resistance gene to penicillin,aminoglycosides,macrolides,tetracycline,vancomycin,and genetic markers of mobile genetic elements play a key role in resistance to antimicrobial agents.

关 键 词：粪肠球菌 耐药 万古霉素 接合型转座酶 样本聚类分析 

分 类 号：R378.1[医药卫生—病原生物学]