三种跳虫肠道菌群的多样性分析及功能预测  

Diversity analysis and function prediction of gut microbiota in three springtail species

作　　者：陈伟 陈霞[2] 李娟[2] 马欣然 崔薇 渠凤甜 谢桂林[1] 赵红庆[2] CHEN Wei;CHEN Xia;LI Juan;MA Xin-Ran;CUI Wei;QU Feng-Tian;XIE Gui-Lin;ZHAO Hong-Qing(College of Life Science,Northeast Agricultural University,Harbin 150036,China;National Institute for Communicable Disease Prevention and Control,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]东北农业大学生命科学学院,哈尔滨150036 [2]中国疾病预防控制中心传染病预防控制所,北京102206

出　　处：《昆虫学报》2020年第4期450-461,共12页Acta Entomologica Sinica

基　　金：黑龙江省教育厅科学技术研究项目(12521001)。

摘　　要：【目的】跳虫在土壤生态系统中发挥着重要的作用。本研究旨在调查Sinella(Coecobrya)oligoseta,Proisotoma minuta和Tomocerus missus 3种跳虫肠道菌群的结构和多样性以及潜在功能。【方法】采用16S rDNA扩增子测序法对以上3种跳虫成虫肠道内容物中的菌群进行分析和比较;应用Tax4Fun法对其肠道菌群基因进行功能预测。【结果】3种跳虫中成虫肠道菌群多样性最高的是T.missus,最低的是S.(C.)oligoseta。在门水平上3种跳虫成虫肠道中最主要的菌群均为变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidetes),放线菌门(Actinobacteria)也具有较高的丰度;在属水平上S.(C.)oligoseta肠道中假单胞菌属Pseudomonas的丰度(16.21%)明显高于P.minuta和T.missus肠道中的丰度(分别为0.87%和1.37%);P.minuta肠道中弧菌属Vibrio的丰度(25.81%)明显高于S.(C.)oligoseta和T.missus肠道中的丰度(分别为3.35%和0.004%)。另外,KEGG pathway注释预测出3种跳虫成虫肠道菌群基因功能涉及碳水化合物和氨基酸代谢、传染性疾病和耐药性。【结论】S.(C.)oligoseta,P.minuta和T.missus这3种跳虫肠道菌群在门水平上的核心菌群相同,而在属水平上的优势菌属丰度存在较大差异,其影响因素应该包括物种自身遗传特点及栖息地环境中微生物种类和数量的不同。在这3种跳虫肠道内发现放线菌,有利于新型放线菌的开发及其代谢产物的利用,并且它们的肠道内很可能存在耐药性和致病性菌株,这为跳虫肠道菌群的功能研究指引了新方向。【Aim】 Springtails make a significant contribution to the soil ecosystem. This study aims to investigate the gut microbial structure and diversity and their potential functions in three springtail species, Sinella(Coecobrya) oligoseta, Proisotoma minuta and Tomocerus missus. 【Methods】 Microbes in the adult intestinal contents of the three springtail species were analyzed and compared by 16 S rDNA amplicon sequencing, and the function prediction of gut microbial genes was conducted using Tax4 Fun method. 【Results】 Among the three springtail species, T. missus showed the highest adult gut microbial diversity, while S.(C.) oligoseta showed the lowest adult gut microbial diversity. At the phylum level, Proteobacteria, Firmicutes and Bacteroidetes were the most predominant microbes in the adult gut microbiota of the three springtail species, followed by Actinobacteria. At the genus level, the abundance of Pseudomonas in the gut of S.(C.) oligoseta(16.21%) was significantly higher than those in the guts of P. minuta(0.87%) and T. missus(1.37%). The abundance of Vibrio in the gut of P. minuta(25.81%) was significantly higher than that in the gut of S.(C.) oligoseta(3.35%) and T. missus(0.004%). Additionally, based on KEGG pathway annotations, we predicted that the genes in adult gut microbiota of the three springtail species may be involved in the metabolism of carbohydrates and amino acids, infectious diseases and drug resistance. 【Conclusion】 In the intestines of the three springtail species, S.(C.) oligoseta, P. minuta and T. missus, the core microbiota are the same at the phylum level, but obviously different at the genus level. The influencing factors should include the genetic traits and the abundance and diversity of microbes in the habitat of each species. Actinomycetes in springtail guts are conducive to the discovery of new strains and the application of their metabolites. Drug-resistant and pathogenic bacteria likely exist in the intestines of the three springtail species, implying new directio

关 键 词：跳虫 肠道菌群 物种多样性 16S RDNA 物种注释 功能预测 

分 类 号：Q965.8[生物学—昆虫学]