Sdr9c7+/-小鼠的生长及高脂饲喂条件下血液生理生化指标分析  

Growth and Effects of High-fat Diet Feed on Blood Physiological and Biochemical Indicators ofSdr9c7+/-Mice

作　　者：曾志棚 周洁[1,2] 卢伟敏 陈俏媛 贺思诺 林万华 ZENG Zhipeng;ZHOU Jie;LU Weimin;CHEN Qiaoyuan;HE Sinuo;LIN Wanhua(Guangxi Universities Key Laboratory of Stem Cell and Biopharmaceutical Technology(Guangxi Normal University),Guilin Guangxi 541006,China;College of Life Sciences,Guangxi Normal University,Guilin Guangxi 541006,China;Affiliated Hospital,Guangxi Normal University,Guilin Guangxi 541006,China)

机构地区：[1]广西高校干细胞与医药生物技术重点实验室(广西师范大学),广西桂林541006 [2]广西师范大学生命科学学院,广西桂林541006 [3]广西师范大学校医院,广西桂林541006

出　　处：《广西师范大学学报（自然科学版）》2020年第3期104-109,共6页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金(31560248);广西自然科学基金(2016GXNSFAA380176);广西师范大学博士科研启动基金;广西研究生教育创新计划(XYCSZ2018055)。

摘　　要：以野生型(Sdr9c7+/+)和杂合子型(Sdr9c7+/-)雄性小鼠为实验动物分析普通饲料和高脂饲料饲喂条件下Sdr9c7基因对小鼠生长发育的影响,并检测高脂饲喂条件下小鼠血液生理生化指标及肝脏脂肪变性情况。结果表明:(1)与Sdr9c7+/+小鼠相比,Sdr9c7+/-小鼠发育正常,二者生长曲线无显著差异;(2)高脂饲料喂养至15周龄时,两组间小鼠血液的葡萄糖(GLU)、血清总胆固醇(TCHO)、甘油三酯(TG)、低密度胆固醇(LDL)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、尿素(BUN)、肌酐(CREA)含量等无差异;至28周龄时,杂合子组小鼠血清的AST和ALT含量显著升高(P<0.05),推测Sdr9c7与肝炎症相关。但肝脏系数和肝脏石蜡组织切片中细胞空泡面积无明显差异,说明Sdr9c7基因对肝脏脂肪变性没有影响。Wild type (Sdr9c7+/+)and heterozygote type (Sdr9c7+/-)male mice were used to analyze the effects ofSdr9c7 gene on the growth and development under regular chow diets and high-fat diets,and the physiological and biochemical indicators of blood and liver steatosis of mice under high-fat diets were also measured.Results:(1)Compared withSdr9c7+/+mice,Sdr9c7+/-mice developed normally,and there was no significant difference in their body weight curves.(2)When the mice fed with high-fat diet to 15 weeks of age,there was no difference in the level of blood glucose(GLU),serum total cholesterol(TCHO),triglyceride(TG),low-density cholesterol(LDL),alanine aminotransferase(ALT),alanine aminotransferase(AST),urea(BUN)and creatinine(CREA)of the mice between the two groups.At the age of 28 weeks under high-fat diets,the AST and ALT levels in the heterozygote group were significantly increased(P<0.05),suggesting thatSdr9c7 might be associated with hepatitis,but there was no significant difference in liver coefficient and cell vacuole area of paraffin section,indicating thatSdr9c7 gene had no effect on hepatic steatosis.

关 键 词：Sdr9c7基因 生长发育 高脂喂食 血液生理生化指标 脂肪肝 

分 类 号：Q592.1[生物学—生物化学]