Proteomic Profiling of Purified Rabies Virus Particles  

作　　者：Yan Zhang Yuyang Wang Ye Feng Zhongzhong Tu Zhiyong Lou Changchun Tu 

机构地区：[1]Key Laboratory of Jilin Province for Zoonosis Prevention and Control,Institute of Military Veterinary Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Changchun 130122,China [2]School of Medicine and Collaborative Innovation Center of Biotherapy,Tsinghua University,Beijing 100084,China [3]Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou 225009,China

出　　处：《Virologica Sinica》2020年第2期143-155,共13页中国病毒学（英文版）

基　　金：This research was funded by the National Key Research and Development Plan(Grant No.2016YFD0500401);National Natural Science Foundation of China(Grant No.31402214);China Postdoctoral Science Foundation(Grant No.2014M552638).

摘　　要：While host proteins incorporated into virions during viral budding from infected cell are known to play essential roles in multiple process of the life cycle of progeny virus,these characteristics have been largely neglected in studies on rabies virus(RABV).Here,we purified the RABV virions with good purity and integrity,and analyzed their proteome by nano LC–MS/MS,followed by the confirmation with immunoblot and immuno-electronic microscopy.In addition to the 5 viral proteins,49 cellular proteins were reproducibly identified to be incorporated into matured RABV virions.Function annotation suggested that 24 of them were likely involved in virus replication.Furthermore,cryo-EM was employed to observe the purified RABV virions,generating high-resolution pictures of the bullet-shaped virion structure of RABV.This study has provided new insights into the host proteins composition in RABV virion and shed the light for further investigation on molecular mechanisms of RABV infection,as well as the discovery of new anti-RABV therapeutics.

关 键 词：Rabies virus(RABV) PURIFICATION Virion structure Virion proteome 

分 类 号：R373[医药卫生—病原生物学]