矽肺病人BALF液miR-146a及IL-1β的表达特征研究  

Expression characteristics of miR-146a and IL-1β in bronchoalveolar lavage fluid collected from silicosis patients

作　　者：李娟[1] 郑晋南 刘云兴 高伟[1] 李茜 张勤[2] LI Juan;ZHENG Jin-nan;LIU Yun-xing;GAO Wei;LI Qian;ZHANG Qin(Second People’s Hospital of Pan Zhi-hua,Pan Zhi-hua,Sichuan 617068,China;不详)

机构地区：[1]攀枝花市第二人民医院,四川攀枝花617068 [2]四川大学华西公共卫生学院/四川大学华西第四医院,四川成都610041

出　　处：《现代预防医学》2020年第10期1852-1855,1920,共5页Modern Preventive Medicine

基　　金：四川省医学会2014年四川省医学科研青年创新课题(Q14035);国家自然科学青年基金(81102107)。

摘　　要：目的探讨不同期别矽肺病人支气管肺灌洗液中miR-146a和IL-1β的表达特征及其相互关系。方法以35名接受支气管肺灌洗治疗的人群为研究对象,采用RT-qPCR法测定研究对象肺灌洗液中miR-146a相对表达量,ELISA法测定IL-1β表达水平。采用t检验、方差分析等方法分析其表达谱特征及其相互关系。结果不同期别矽肺病人肺灌洗液miR-146a及IL-1β表达水平均呈现先上升后下降的趋势,其中,miR-146a相对表达量在贰期达到最高水平(P<0.05),IL-1β表达量在壹期达最高水平(P<0.05),并且壹期、贰期矽肺患者miR-146a及IL-1β的表达水平均高于对照组(P<0.05);肺灌洗液miR-146a相对表达量与IL-1β表达水平的相关系数为0.457(P<0.05)。结论不同期别矽肺病人肺灌洗液中miR-146a与IL-1β的表达水平不同,二者之间存在相关性,提示miR-146a主要在矽肺早期发挥调控作用,并且表达水平与初次接尘年龄、工龄、饮酒等有关。Objective To discuss the expression characteristics and correlation of miR-146a and IL-1βin silicosis patients of different stages.Methods 35 volunteers experienced bronchoalveolar lavage therapy were enrolled.And then,the expression level of miR-146a and IL-1βin macrophages of bronchoalveolar lavage fluid were determined by RT-qPCR and ELISA,respectively.Analysis of variance,t-test and other statistical methods were used to study the expression profiling of miR-146a and IL-1βand their correlation.Results The expression levels of miR-146a and IL-1βin macrophage in lavage fluid of silicosis patients in different stages were different,but both of which showed a rising first and then declining trend.The relative expression levels of miR-146a and IL-1βreached the highest level in stage II and stage I respectively,with statistically significant differences(P<0.05),and both of them in stage I and II silicosis patients were higher than those in control group(P<0.05).There was a positive correlation(r=0.457)between the expression levels of miR-146a and IL-1β(P<0.05).Conclusion The expression levels of miR-146a and IL-1βin lavage fluid of silicosis patients in different stages are different.At the same time,the expression level of IL-1βis correlated with the expression of miR-146a.Result indicates that miR-146a plays a regulatory role in the early stage of silicosis and its expression level is related to the initial age of dust exposure,working years,drinking,etc.

关 键 词：矽肺 MIR-146A IL-1Β 肺灌洗液 

分 类 号：R135.2[医药卫生—劳动卫生]