南繁地区筛网用于转基因玉米种植隔离距离控制条件的研究  

Using Screen Mesh to Control the Isolation Distance of Transgenic Maize Planted in Off-Season Reproduction Regions

作　　者：谭燕华[1] 谢翔[1] 周霞[1] 易小平[1] 霍姗姗[1] 张丽丽[1] 曹扬[1] 赵辉[1] 郭安平[1] TAN Yanhua;XIE Xiang;ZHOU Xia;YI Xiaoping;HUO Shanshan;ZHANG Lili;CAO Yang;ZHAO Hui;GUO Anping(Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences/Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions,Haikou,Hainan 571101,China)

机构地区：[1]中国热带农业科学院热带生物技术研究所/海南省南繁生物安全与分子育种重点实验室,海南海口571101

出　　处：《热带作物学报》2020年第5期851-858,共8页Chinese Journal of Tropical Crops

基　　金：转基因生物新品种培育科技重大专项(No.2016ZX08012005);中国热带农业科学院基本科研业务费专项(No.1630052017013)。

摘　　要：筛网作为控制基因漂移的物理隔离屏障早已应用在农业生产中,然而一直缺乏可靠的试验数据供选择。因此,本研究使用筛网作为隔离措施,研究南繁地区转基因玉米基因漂移距离的控制条件,研究期为2 a,分别在2个南繁季节和2个南繁地点进行。结果显示:在基因漂移阈值为0.1%的条件下,250目的筛网在高度高于种植的玉米2.5 m时,可有效地将花粉漂移距离控制在20~30 m,大大缩短了转基因玉米安全监管规定的隔离距离,这对于农用土地越来越少的南繁基地是非常有利的。本研究结果也可适用于南繁地区常规玉米制种过程的基因漂移风险控制。Screen mesh has been used as a physical barrier isolation material to control the pollen flow in agricultural production for a long time, but there has been little reliable experimental data for the application. A physical isolation facility using screen mesh to isolate transgenic maize from non-transgenic maize was studied for two seasons 2015 and 2016. and at two different planting areas in Hainan Island. Screen mesh with 250 mesh and height of 2.5 m above the height of transgenic maize was good enough to prevent pollen flow at a critical distance of 20–30 m, when the threshold valve of genetic drift rate was set as 0.1%, which greatly shortened the isolation distance stipulated by the safety control of GM maize, and this was quite beneficial for Off-Season breeding due to the dwindling agricultural land. The study is also applicable to the gene flow risk control of traditional crops in Off-Season breeding areas during seed production process.

关 键 词：筛网 转基因玉米 基因漂移 隔离控制 南繁 

分 类 号：S513[农业科学—作物学]