小飞蓬捕光蛋白CcLhca-Z6基因克隆及羊脂酸胁迫下的表达分析  

Cloning Analysis of Light-harvesting Protein CcLhca-Z6 Gene in Conyza canadensis and Its Response to Caprylic Acid Stress

作　　者：徐晶晶 苑平 柏浩东 廖海民[1] 李祖任[1,2] XU Jingjing;YUAN Ping;BAI Haodong;LIAO Haimin;LI Zuren(Colleges of Life Sciences,Guizhou University,Guiyang,Guizhou 550025;Hunan Prorincial Key Laboratory for Biology and Control of Weeds,Hunan Academy of Agriculural Sciences,Changsha,Hunan 410125)

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]湖南省农业科学院,杂草生物学及安全防控生物学湖南省重点实验室,湖南长沙410125

出　　处：《核农学报》2020年第5期932-938,共7页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(31801754);湖南省自然科学基金(2018JJ3289);湖南省农业科技创新资金项目(2017-GC-01)

摘　　要：为了探究羊脂酸对小飞蓬的化感作用,以羊脂酸处理小飞蓬的蛋白质组学数据信息为基础,采用同源克隆和RACE技术克隆了编码CcLhca-Z6基因的完整cDNA序列,利用荧光定量PCR比较了羊脂酸处理后不同时间CcLhca-Z6表达量的差异。结果表明,CcLhca-Z6基因序列全长1016 bp(Gene Bank序列号为MH512007.1),编码266个氨基酸,分子量为28.14 kDa,理论等电点为5.29;小飞蓬Lhca-Z6蛋白与黄花蒿(PWA84283.1)蛋白进化程度最为接近,处于同一分支,亲缘比较近。RT-qPCR分析结果显示,羊脂酸处理显著影响小飞蓬叶片CcLhca-Z6基因的表达,并随着处理时间的延长呈现先升高后降低的趋势。由此推测,CcLhca-Z6基因可能是小飞蓬响应羊脂酸胁迫的关键基因。本研究结果为进一步探索植物源除草剂羊脂酸的作用机理奠定了基础。To explore the allelopathic effect of caprylic acid on Conyza canadensis, based on the proteomics data of C. canadensis under caprylic acid treatment, the complete cDNA sequence of CcLhca-Z6 gene was cloned by homologous cloning and RACE technology. The expression of CcLhca-Z6 gene was measured by fluorescence quantitative PCR at different time after caprylic acid application. The results showed that the full length of CcLhca-Z6 was 1016 bp(Gene Bank NO. MH512007.1), encoding 266 amino acid residues with a molecular mass of 28.14 kD and theoretical pI of 5.29. Phylogenetic analysis showed that CcLhca-Z6 protein was clustered into a group with Artemisia annua(PWA84283.1), and the CcLhca-Z6 protein had the closest genetic relationship with Artemisia annua(PWA84283.1). RT-qPCR results showed that the treatment with caprylic acid significantly affected the expression of Lhca-z6 gene in the leaves of C. canadensis which showed a trend of first increasing and then descending with time. It was speculated that the CcLhca-Z6 gene may be the key gene in response to caprylic acid stress. This study laid a foundation for further exploring the function mechanism of plant herbicide caprylic acid.

关 键 词：小飞蓬 羊脂酸 光捕获蛋白 基因克隆 基因表达 

分 类 号：S451[农业科学—植物保护]