流式细胞术在兰科植物中的应用  

Applications of Flow Cytometry in Orchidaceae

作　　者：李春楠[1] 傅巧娟[1] 沈国正[1] 赵福康[1] 张晓莹 阮若昕 LI Chunnan;FU Qiaojuan;SHEN Guozheng;ZHAO Fukang;ZHANG Xiaoying;RUAN Ruoxin(Hangzhou Aecdemy of Agriculural Sciences,Hangzhou,Zhejiang 310024)

机构地区：[1]杭州市农业科学研究院,浙江杭州310024

出　　处：《核农学报》2020年第5期973-981,共9页Journal of Nuclear Agricultural Sciences

基　　金：杭州市科技发展计划项目(20180432B23)。

摘　　要：兰科是重要的观赏、药用园艺植物,在长期的进化演变中形成了丰富的形态类型和复杂的染色体倍性,并普遍存在内多倍性现象。挖掘兰科的遗传信息是育种研究中重要的基础工作。流式细胞术作为一种高通量的细胞分析和分选技术,在植物DNA含量、细胞周期和倍性鉴定等方面应用广泛。本文主要综述流式细胞术在兰科基因组大小测定、染色体倍性分析和内多倍性研究等方面的应用,为进一步利用流式细胞术开展兰科植物杂交育种、染色体结构、进化分类等相关研究提供参考。As an important ornamental and medicinal horticultural plant, Orchidaceae exhibit diverse morphological types, complex chromosome ploidy, and commonly appear endopolyploidization during the long-term evolution. Exploring the genetic information of Orchidaceae is important for breeding research. Flow cytometry(FCM) is a high-throughput technique for cell analysis and separation, which has been extensively applied in detecting plant DNA content, cell cycle and ploidy level. This paper mainly reviewed the current applications of flow cytometry in genome-size, chromosome ploidy and endopolyploidy in Orchidaceae, which might provide references for its further applications in plant crossbreeding, chromosome structure and evolutionary classification, and etc.

关 键 词：流式细胞术 兰科 基因组大小 染色体倍性 内多倍性 

分 类 号：S682.31[农业科学—观赏园艺]