非梗阻性无精子症患者睾丸组织CREM表达及意义  

The expression of CREM gene in testicular tissue of the patients with non-obstructive azoospermia

作　　者：刘金 于红玲[1] 孟凡伟[1] Liu Jin;Yu Hongling;Meng Fanwei(Center for Reproductive Medicine,Department of Obstetrics and Gynecology,Qilu Hospital,Cheeloo College of Medicine,Shandong University,Jinan 250012)

机构地区：[1]山东大学齐鲁医院妇产科不孕不育诊疗中心,济南250012

出　　处：《现代妇产科进展》2020年第6期438-440,共3页Progress in Obstetrics and Gynecology

摘　　要：目的:探讨非梗阻性无精子症(NOA)患者睾丸组织中CREM表达及意义。方法:选取NOA患者28例和生精正常的梗阻性无精子症(OA)患者19例,收集睾丸活检组织,采用RT-PCR、免疫组化法及Western blot法检测睾丸组织中CREM mRNA及CREM蛋白表达。结果:CREM蛋白主要表达于睾丸组织生精小管的精母细胞、精原细胞和早期精子细胞的细胞核。OA睾丸组织中CREM mRNA含量高于NOA睾丸组织[(0.028±0.022)vs(0.014±0.024)],OA睾丸组织中CREM蛋白表达高于NOA睾丸组织[(2.263±0.371)vs(1.145±0.535)],差异均有统计学意义(P<0.001)。结论:睾丸组织中CREM mRNA及蛋白表达下调可能是NOA发病的原因之一。Objective:To investigate the expression of CREM gene in testicular tissue of the patients with non-obstructive azoospermia.Methods:19 cases of obstructive azoospermia testicular tissue and 28 cases of non-obstructive azoospermia testicular tissue were collected.The expression of CREM gene was detected using immunohistochemical,reverse transcription-polymerase chain reaction(RT-PCR)and Western blot.Results:The CREM protein was mainly expressed on round spermatids,spermatogones and spermatocytes of seminiferous tubule in testicular tissue.The CREM mRNA expression in OA testicular tissue was higher than that in NOA testicular tissue[(0.028±0.022)vs(0.014±0.024)],the CREM protein expression was higher than that in NOA testicular tissue[(2.263±0.371)vs(1.145±0.535)],the difference was statistically significant(P<0.001).Conclusion:The reduced expression of CREM may be one of the cause of the NOA.

关 键 词：CREM 人睾丸组织 生精功能 

分 类 号：R698[医药卫生—泌尿科学]