碱性苯酚和卫可消毒剂对结核分枝杆菌消毒效果验证  

Validation of disinfection effect of Vesphene IIse and VirkonS disinfectant on Mycobacterium tuberculosis

作　　者：杨孝祥 曾志强 蒋国斌 刘威龙[1] 张明霞[1] YANG Xiaoxiang;ZENG Zhiqiang;JIANG Guobin;LIU Weilong;ZHANG Mingxia(Guangdong Key Laboratory of Emerging Infectious Disease,The Third People′s Hospital of Shenzhen,Shenzhen,Guangdong 518112,China)

机构地区：[1]深圳市第三人民医院广东省新发传染病重点实验室,广东深圳518112

出　　处：《中国热带医学》2020年第5期477-479,共3页China Tropical Medicine

基　　金：国家科技重大专项(No.2017ZX10201301-001-002);国家自然科学基金面上项目(No.81671984);深圳医疗卫生“三名工程”(No.SZSM201412001)。

摘　　要：目的验证碱性苯酚和卫可消毒剂对结核分枝杆菌的杀菌能力,以寻求结核菌实验室最佳消毒效果。方法悬液定量杀灭试验测定5、10、20 min,浓度为1∶85、1∶128、1∶256的碱性苯酚消毒剂和浓度为1∶66、1∶100、1∶133、1∶200、1∶400的卫可消毒剂对结核分枝杆菌的杀菌能力。各试验重复3次。结果1∶85碱性苯酚消毒剂消毒5 min可以完全杀灭结核分枝杆菌,但是消毒10、20 min不能完全杀灭结核分枝杆菌;1∶128消毒5 min内不能完全杀灭结核分枝杆菌,消毒10 min和20 min可以完全杀灭结核分枝杆菌;1∶256碱性苯酚消毒剂消毒5、10、20 min均不能完全杀灭结核分枝杆菌;而1∶66、1∶100、1∶133、1∶200和1∶400的卫可消毒剂消毒5、10、20 min均不能完全杀灭结核分枝杆菌。结论浓度为1∶128的碱性苯酚消毒剂作用10 min可以有效杀灭结核分枝杆菌,可以作为结核实验室消毒剂;卫可消毒剂虽为广谱消毒剂但对结核分枝杆菌不敏感,杀灭效率差,不可作为结核实验室消毒剂。Objective To verify the bactericidal ability of VespheneⅡse and Virkon S disinfectant againstMycobacterium tuberculosis,and we seek the best disinfection effect of tuberculosis laboratory.Methods Suspension quantitative germicidal test was used to determine the germicidal efficacy of 5,10 and 20 minutes of 1∶85,1∶128,1∶256 VespheneⅡse disinfectant and 1∶66,1∶100,1∶133,1∶200,1∶400 Virkon S disinfectant against Mycobacterium tuberculosis.Each test was repeated 3 times.ResultsThe 1∶85 concentration VespheneⅡse disinfectant can kill Mycobacterium tuberculosis within 5 minutes,but can not completely kill Mycobacterium tuberculosis within 10,20 minutes;1∶128 concentration disinfection can not completely kill Mycobacterium tuberculosis within 5 minutes,disinfection can completely killMycobacterium tuberculosis within 10 minutes and 20 minutes;1∶256 concentration VespheneⅡse disinfectant can notcompletely kill Mycobacterium tuberculosis within 5,10,and 20 minutes;while 1∶66,1∶100,1∶133,1∶200 and 1∶400 concentration Virkon S disinfectant can not completely kill Mycobacterium tuberculosis within 5,10,and 20 minutes.Conclusion The Vesphene IIse disinfectant with a concentration of 1∶128 can effectively kill Mycobacterium tuberculosis andcan be used as a disinfectant in the tuberculosis laboratory for 10 minutes;the Virkon S disinfectant,though a broad-spectrum disinfectant,is not sensitive to Mycobacterium tuberculosis and has poor killing efficiency,so it can not be used as a disinfectantin the tuberculosis laboratory.

关 键 词：结核分枝杆菌 碱性苯酚消毒剂 卫可消毒剂 

分 类 号：R378.911[医药卫生—病原生物学]