HBV准种测序技术应用及抗病毒药物治疗对HBV准种变异影响的研究进展  

The Advances in the Application of HBV Quasispecies Sequencing Technology and Antiviral Drug Therapy for the Quantification of HBV Quasispecies

作　　者：文凤 金子铮 刘宁[1] 娄金丽[1] WEN Feng;JIN Zizheng;LIU Ning;LOU Jinli(Beijing You′an Hospital,Capital Medical University Laboratory, Beijing 100069, China)

机构地区：[1]首都医科大学附属北京佑安医院检验科,北京100069

出　　处：《标记免疫分析与临床》2020年第4期697-701,共5页Labeled Immunoassays and Clinical Medicine

基　　金：佑安肝病艾滋病基金(编号:YNKTTS20180104);国家科技重大专项(编号:2018ZX10305-410-006)。

摘　　要：乙型肝炎(hepatitis B)大多数是由嗜肝DNA病毒科正嗜肝病毒属病毒感染人体所致,其乙型肝炎病毒具有高突变、高复制的特点,在人体体内以准种的形式存在。乙型肝炎病毒不仅可以引起隐匿性、急慢性肝炎,还可导致肝硬化(liver cirrhosis),与肝癌(HCC)的发生发展也密切相关。对HBV准种测序应用的选择以及抗病毒治疗后HBV准种变异情况对乙型肝炎的发病机制、疾病转归以及疗效预测有着重要意义。通过高效HBV测序技术的应用,了解HBV准种突变的靶点,进行精准靶向治疗从而提高治疗效果,达到临床治愈的目的。本文就测序技术的应用及抗病毒药物治疗对HBV准种变异的影响作一综述。Hepatitis B virus(HBV)is a hepadnavirus(DNA)virus with high replication rate and high mutation rate.It exists in the host in the form of quasispecies.HBV infection can not only cause occult,acute and chronic viral hepatitis,but also closely related to the occurrence and development of cirrhosis and hepatocellular carcinoma(HCC).The selection of HBV quasispecies sequencing methods and evaluation of HBV quasispecies variation after antiviral treatment are important for the understanding of the pathogenesis of hepatitis,the outcome of the disease,and the prediction of efficacy.Through the efficient use of HBV quasi-species sequencing,researcher can understand the variation characteristics of hepatitis B virus,which can lead to the use of targeted antiviral drugs to inhibit the replication of hepatitis B virus,so to achieve clinical treatment.This article reviews the application of sequencing technology and the impact of antiviral drug therapy on HBV quasispecies variation.

关 键 词：乙型肝炎病毒 HBV准种 测序 抗病毒治疗 

分 类 号：R373.2[医药卫生—病原生物学]