低硒条件下T-2毒素对大鼠关节软骨及骨髓成纤维细胞生长因子8和成纤维细胞生长因子受体3表达的影响  

Effects of T-2 toxin on expression of fibroblast growth factor 8 and fibroblast growth factor receptor 3 in articular cartilage and bone marrow of rats under low selenium condition

作　　者：史敏[1] 方倩 石亚雯 米鸽 李达宁 王会 王梦莹 张萌 马天有 陈静宏 Shi Min;Fang Qian;Shi Yawen;Mi Ge;Li Daning;Wang Hui;Wang Mengying;Zhang Meng;Ma Tianyou;Chen Jinghong(Department of Pathology,School of Medicine,Xi'an Peihua University,Xi'an 710125,China;Institute of Endemic Diseases,School of Public Health,Xi'an Jiaotong University Health Science Center,Key Laboratory of Trace Elements and Endemic Diseases of National Health Commission,Xi'an 710061,China)

机构地区：[1]西安培华学院医学院病理教研室,710125 [2]西安交通大学医学部公共卫生学院地方病研究所国家卫健委微量元素与地方病重点实验室,710061

出　　处：《中华地方病学杂志》2020年第2期79-85,共7页Chinese Journal of Endemiology

基　　金：西安培华学院2019年度校级科研项目(PHKT19008);国家自然科学基金(81573102、81872565)。

摘　　要：目的了解低硒条件下T-2毒素对大鼠关节软骨及软骨下骨髓成纤维细胞生长因子8(FGF8)和成纤维细胞生长因子受体3(FGFR3)表达的影响,探讨其在大骨节病(KBD)软骨深层损伤和继发性并发症中的作用机制.方法选取24只健康雄性SD大鼠(体质量为60~80 g),采用随机数字表法分为常规饲料组(硒含量101.5μg/kg)和低硒饲料组(硒含量1.1μg/kg),每组12只.饲养30 d后,将常规饲料组分为对照组和T-2毒素组(100μg·kg-1·d-1),低硒饲料组分为低硒组和低硒+T-2毒素组,每组6只.继续饲养30 d处死大鼠,取膝关节软骨附带松质骨,HE染色观察膝关节软骨病理学改变;免疫组化法检测膝关节软骨及软骨下骨髓FGF8和FGFR3表达情况,计算关节软骨FGF8和FGFR3阳性表达率,并通过Image-Pro Plus 6.0软件测定软骨下骨髓FGF8和FGFR3阳性表达积分光密度(IOD)值.结果光镜下,低硒+T-2毒素组软骨细胞稀疏,关节软骨深层和中层可见空的软骨细胞囊增多,软骨细胞死亡成为红染的细胞影子,深层区域内细胞外基质降解而淡染,成为坏死无结构区,附近可见增生的肉芽组织.低硒+T-2毒素组大鼠关节软骨FGF8阳性表达率[(88.61±10.97)%]高于对照、低硒、T-2毒素组[(10.35±2.48)%、(19.26±3.08)%、(58.89±9.29)%,P均<0.05],软骨下骨髓FGF8阳性表达IOD值[(16.73±1.72)×106]高于对照、低硒、T-2毒素组[(1.20±0.41)×106、(4.33±0.97)×106、(12.80±1.12)×106,P均<0.05].低硒+T-2毒素组大鼠关节软骨FGFR3阳性表达率[(89.76±8.59)%]高于对照、低硒、T-2毒素组[(13.18±2.25)%、(21.15±2.33)%、(32.55±6.72)%,P均<0.05],软骨下骨髓FGFR3阳性表达IOD值[(16.50±5.36)×106]高于对照、低硒、T-2毒素组[(7.58±1.02)×106、(10.73±7.13)×106、(9.83±5.63)×106,P均<0.05].结论在低硒条件下T-2毒素改变了大鼠关节软骨深层及软骨下骨髓FGF8和FGFR3的表达,FGF8和FGFR3的高表达可能参与KBD继发性改变的发生和发展.Objective To study the effects of T-2 toxin on expression of fibroblast growth factor 8(FGF8)and fibroblast growth factor receptor 3(FGFR3)in articular cartilage and subchondral marrow of rats under low selenium condition,and to explore the mechanism of deep cartilage injury and secondary complications in Kaschin-Beck disease(KBD).Methods Twenty-four healthy male SD rats weighted 60-80 g were selected,they were divided into conventional feed group(selenium content of 101.5μg/kg)and low-selenium feed group(selenium content of 1.1μg/kg)by random number table method,with 12 rats in each group.After 30 days of feeding,the conventional feed group was further divided into control group and T-2 toxin group(100μg·kg-1·d-1),and the low-selenium feed group was further divided into low-selenium group and low-selenium+T-2 toxin group,with 6 rats in each group.After 30 days of feeding,the rats were sacrificed and the knee cartilage with cancellous bone was taken.Pathological changes of knee cartilage were observed by HE staining.Immunohistochemical method was used to detect the expression of FGF8 and FGFR3 in cartilage and subchondral marrow of knee joint,positive expression rates of FGF8 and FGFR3 in articular cartilage were calculated,and the integrated optical density(IOD)values of FGF8 and FGFR3 positive expression in subchondral marrow were analyzed by Image-Pro Plus 6.0 software.Results Under light microscope,chondrocytes in low-selenium+T-2 toxin group were sparse,and empty chondrocytes in the deep and middle layers of articular cartilage increased,and chondrocytes died and became red cell shadows.The extracellular matrix dissolved and was slightly stained in deep region,turning into necrotic and unstructurized areas.Proliferating granulation tissue was visible nearby.The positive expression rate of FGF8 in articular cartilage of rats in low-selenium+T-2 toxin group[(88.61±10.97)%]was higher than that in control,low-selenium and T-2 toxin groups[(10.35±2.48)%,(19.26±3.08)%,(58.89±9.29)%,P<0.05];IOD value of FG

关 键 词：硒 T-2毒素 大鼠 大骨节病 成纤维细胞生长因子8 成纤维细胞生长因子受体3 

分 类 号：R6[医药卫生—外科学]