蒙药三味阿拉坦其其格散的急性毒性试验  

Acute Toxicity Test of Mongolian Medicine Alatanqicjige-3

作　　者：特日格乐 苏龙嘎 齐和日玛[1] 兴安 包纳日斯[1] 萨仁高娃[1] Terigele;Sulongga;Qiherima;Xingan;BAO Na ri si;Sarengaowa(Inner Mongolia Medical University,Hohhot 010110,China)

机构地区：[1]内蒙古医科大学,内蒙古呼和浩特01011

出　　处：《中国民族民间医药》2020年第10期36-40,共5页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：内蒙古自然科学基金面上基金(No.2019MS08156);内蒙古医科大学蒙药学“一流学科”青年教师创新能力提升项目(No.myxylxkky2019-01)。

摘　　要：目的:通过观察蒙药方剂三味阿拉坦其其格对小鼠的急性毒性反应和动物死亡情况,对三味阿拉坦其其格进行安全性评价,力求为其临床用药提供安全范围。方法:按照经典急性毒性实验方法进行,观察小鼠中毒反应及计算出半数致死量(LD50),给药后连续观察7 d,实验结束后处死小鼠,观察重要器官是否存在病理改变。结果:蒙药方剂三味阿拉坦其其格LD50为16.03 g·kg-1,95%置信区间(CI)为11.38~22.60 g·kg-1。实验过程中小鼠体质量在给药3 d后恢复正常,组织病理切片显示,肝脏空泡变性、椭圆形细胞增生病变、脾脏淋巴细胞凋亡等病变可能与药物使用有关。结论:实验表明蒙药方剂三味阿拉坦其其格具有一定的急性毒性,但在一定剂量范围内使用是安全的。Objective By observing the acute toxicity and animal death of Mongolian medicine Alatanqiqige-3 to mice,to evaluate the safety of Alatanqiqige-3 in order to provide a safe range for its clinical use.Methods According to the classical acute toxicity experiment method,observe the toxic reaction of mice and calculate the Median lethal dose(LD50).After administration,the mice were observed for 7 days continuously.After the experiment,the mice were killed and the pathological changes of important organs were observed.Results Mongolian medicine Alatanqiqige-3’s LD50 was 16.03 g kg-1 and 95%confidence interval(Cl)was 11.38-22.60 g kg -1.During the experiment,the body mass of mice returned to normal after threedays of administration.The histopathological sections showed that the liver vacuolar degeneration,oval cell hyperplasia,spleen lymphocyte apoptosis and other lesions may be related to the use of drugs.Conclusion The experiment shows that the Mongolian medicine Alatangqige-3 has certain acute toxicity,but it is safe to use in a certain dose range.

关 键 词：蒙药方剂三味阿拉坦其其格 小鼠 急性毒性实验 

分 类 号：R285.1[医药卫生—中药学]