生防菌链霉菌对西瓜枯萎病防治及幼苗生长的影响  

Biocontrol and Plant Growth Promoting Effects of Streptomyces against Fusarium Wilt in Watermelon

作　　者：孙洪宝 李茂营 吴慧玲[3] 郭绍贵[2] 张洁[2] 任毅[2] 张海英[2] 宫国义[2] 许勇[2] SUN Hong-bao;LI Mao-ying;WU Hui-ling;GUO Shao-gui;ZHANG Jie;REN Yi;ZHANG Hai-ying;GONG Guo-yi;XU Yong(Plant Science and Technology College,Beijing University of Agriculture,Beijing 102206,China;National Engineering Research Center for Vegetables,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;Institute of Plant and Environment Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China)

机构地区：[1]北京农学院植物科学技术学院,北京102206 [2]北京市农林科学院蔬菜研究中心,国家蔬菜工程技术研究中心,北京100097 [3]北京市农林科学院植物保护环境保护研究所,北京100097

出　　处：《科学技术与工程》2020年第13期5074-5079,共6页Science Technology and Engineering

基　　金：国家重点研发计划项目(2018YFD0100703);北京市农林科学院科技创新能力建设专项(KJCX20200521);国家西甜瓜产业技术体系项目(CARS-25);国家自然科学基金(31801885);北京西甜瓜创新团队项目(BAIC10-2020)。

摘　　要：西瓜枯萎病作为一种土传真菌病害,严重危害西瓜生产,利用生物防治技术提高抗病性对西瓜安全生产具有重要意义。利用利迪链霉菌(Streptomyces lydicus)A02作为生防菌处理接种西瓜枯萎病菌生理小种1(Fusarium oxysporum f.sp.niveurn race1,FON1)的西瓜幼苗,探究生防菌对西瓜枯萎病的防控作用。结果表明:链霉菌A02对西瓜枯萎病防治效果可达68.23%。枯萎病菌FON1侵染条件下,接种链霉菌A02可增加西瓜幼苗株高、茎粗、鲜重和干重;显著提高西瓜幼苗叶片净光合速率、细胞间CO 2浓度、气孔导度、蒸腾速率及叶绿素含量;与单接种FON1相比,接种链霉菌A02可使水杨酸(salicylic acid,SA)含量增加62.00%。综上,链霉菌A02可通过提高净光合速率和植物内源激素含量,提高西瓜对枯萎病的抗性。Fusarium wilt is a soil-borne fungal disease in watermelon,and it seriously limits watermelon production.Application of biological control technology is called to enhance the disease tolerance of watermelon wilt for watermelon harvest.Therefore,Streptomyces lydicus A02(A02)was used as a biocontrol agent to treat the disease against invasion of Fusarium oxysporum f.sp.race 1(FON1).Results show that the control efficiency of S.lydicus A02 against Fusarium wilt of watermelon reached 68.23%.After inoculation with A02 for FON1-infected watermelon,the height,stem diameter,fresh weight,and dry weight of watermelon seedlings were prompted.Meanwhile,the netphotosynthetic rate,intercellular CO 2 concentration,stomatal conductance,transpiration rate,and chlorophyll content of watermelon seedlings were also increased remarkably,and salicylic acid(SA)content increased by 62.00%over that without A02 treatment.Therefore,A02 can improve the resistance of watermelon against Fusarium wilt by increasing the net photosynthetic rate and planthormone content.

关 键 词：西瓜 枯萎病 链霉菌A02 生物防治 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]