挤奶厅气载大肠杆菌的分离鉴定及其REP-PCR指纹图谱分型研究  

Use of REP-PCR to Identify and Analyze the DNA Fingerprints of Airborne E.coli in the Milk Hall

作　　者：钟召兵 王宁[2] 孙红华[2] 杨夫会[2] ZHONG Zhao-bing;WANG Ning;SUN Hong-hua;YANG Fu-hui(Taian Daiyue District Administrative Examination and Approval Service Bureau,Taian 271018;Taian Daiyue District Animal Husbandry and Veterinary Bureau,Taian 271018)

机构地区：[1]山东省泰安市岱岳区行政审批服务局,泰安271018 [2]山东省泰安市岱岳区畜牧兽医局,泰安271018

出　　处：《中国奶牛》2020年第4期1-4,共4页China Dairy Cattle

基　　金：泰安市科技计划项目“奶牛高产高效繁育技术集成示范”[2016]。

摘　　要：为了解生鲜乳收购站挤奶厅空气中气载大肠杆菌污染情况及不同菌株间遗传相似性,为生鲜乳日常生产实时控制大肠杆菌提供有效技术方法,采用国际标准ANDERSEN-6级空气微生物样品收集器从挤奶厅空气中收集大肠杆菌,利用大肠杆菌DNA基因外重复一致回文序列聚合酶链式反应(Repetitive Extragenic Palindromic elements,PCR,REP-PCR)鉴定技术,对分离菌株进行分子多样性分析。共采集95份样品,从23份中分离到大肠杆菌16株。REP-PCR把分离菌株分为13种基因型(A-M),只有6株菌两两同源性≥90%。结果显示,挤奶厅空气中存在大肠杆菌污染,基因型多且同源性较低,需要采取措施从源头上控制其散播。To determine the prevalence of E.coli infection in fresh milk and genetic similarities between different strains and to then provide an effective technological support for the real-time monitoring Airborne E.coli in the dairy production in the milk hall.The air samples were collected using Andersen-6 stages sampler in the milk hall.and a conventional culture-based study was performed and E.coli strains according to the standard method were isolated and identified.in this study.The(repetitive extragenic palindromic elements,PCR)REP-PCR method was applied to generate genomic amplification products of airborne E.coli isolated the air.then their DNA fingerprints were analyzed.E.coli was detected in 23/95 samples and 16 E.coli strains were obtained,The fingerprints and the phylogenetic tree indicated that The isolates were divided into 13 genotypes(A-M),and only 6 strains had more than 90%homology.The results showed that There were many genotypes and low homology of E.coli in the air of the milking hall.Measures should be taken to control the spread of E.coli from the source.

关 键 词：挤奶厅 气载大肠杆菌 分离鉴定 REP-PCR 

分 类 号：S858.23[农业科学—临床兽医学]