奶牛胚胎早期死亡的转录组初探  

Transcriptome Analysis of Early Embryo Death in Dairy Cattle

作　　者：吕小青[1,2,3] 刘林 薛建华[1,2,3] 王彦平 李艳华[1,2,3] 麻柱 LV Xiao-qing;LIU Lin;XUE Jian-hua;WANG Yan-ping;LI Yan-hua;MA Zhu(Beijing Dairy Cattle Center,Beijing 100192;Key Laboratory of Dairy Cattle Center Breeding and Reproduction,Beijing 100192;Beijing Key Laboratory of Dairy Cattle Genetic,Breeding and Reproduction,Beijing 100192)

机构地区：[1]北京奶牛中心,北京100192 [2]农业农村部奶牛遗传育种重点实验室,北京100192 [3]奶牛遗传育种与繁殖北京市重点实验室,北京100192

出　　处：《中国奶牛》2020年第5期23-25,共3页China Dairy Cattle

基　　金：北京市科技计划课题“奶牛良种扩繁关键技术研究与推广应用(D171100002417002)”;现代奶牛产业技术体系北京市创新团队岗位专家(BAIC06-2019)

摘　　要：为了探讨奶牛胚胎早期死亡的分子机理,通过对奶牛8-细胞期和桑葚胚期死亡和正常的胚胎样本进行基因转录组测序,联合分析不同时期胚胎之间的差异表达基因,挖掘奶牛胚胎早期死亡的关键基因。本研究首先对单个卵裂球的mRNA进行了提取和反转录,其次成功建立了cDNA文库,并用Illumina高通量测序系统完成胚胎的测序,最后分析了不同时期胚胎的表达差异基因。得到如下结果:①成功利用单细胞RNA提取体系建立并扩增了不同时期胚胎的cDNA文库;②对8-细胞期差异表达基因进行分析,获得2倍以上差异基因157个,其中上调基因6个,下调基因151个;对桑葚胚期差异基因进行分析,获得2倍以上差异基因269个,其中上调基因10个,下调基因259个;③通过GO富集分析发现,正常胚胎和死亡胚胎的差异基因表现在细胞、繁殖过程和结合等方面;④通过KEGG分析,8-细胞期和桑葚胚期正常胚胎与死亡胚胎的差异基因主要表现在细胞凋亡信号通路和内质网蛋白合成通路上。In order to explore the molecular mechanism of early embryo death in dairy cows,we sequenced the gene transcriptome of 8-cell and morula embryo samples,analyzed the differentially expressed genes in different embryos,and mined the key genes of early embryo death in dairy cattle.We first extracted and reversed transcribe the mRNA of a single blastomere,then successfully established a cDNA library,and completed the sequencing of embryos with Illumina high-throughput sequencing system,and finally analyzed the gene expression differences in different stages of embryos.The results are as follows:①the cDNA library of embryos in different stages was successfully established and expanded by using single cell RNA extraction system;②totally 157 genes with more than 2 times differential expression were detected in 8-cell stage,including 6 genes with up-regulation and 151 genes with down-regulation;269 genes with more than 2 times differential expression were detected in morula stage,including 10 genes with up-regulation and 259 genes with down-regulation;③by go enrichment analysis,it is found that the differential genes of normal embryo and dead embryo are expressed in the aspects of cell process,reproduction and combination;④by KEGG analysis,the differential genes of normal embryo and dead embryo in 8-cell stage and morula stage are mainly expressed in the apoptotic signal pathway and endoplasmic reticulum protein synthesis pathway.

关 键 词：奶牛 体外受精 早期胚胎 转录组 

分 类 号：S823.3[农业科学—畜牧学]