携hVEGF165表达载体阳离子脂质微泡的制备  

Preparation of cationic lipid microbubbles containing hVEGF165 expression vector.

作　　者：伍巧玲 丁云川 冉海涛[3] 王志刚[3] 陈剑 王庆慧 张键 Wu Qiaoling;Ding Yunchuan;Ran Haitao(Kunming Medical University;Yan'an Hospital Department of Ultrasound,Kunming Yunnan 650051,China;Institute of ultrasound imaging,Chongqing Medical University,Chongqing 400010,China)

机构地区：[1]昆明医科大学 [2]昆明市延安医院超声医学科,云南昆明650051 [3]重庆医科大学超声影像学研究所,重庆400010

出　　处：《云南医药》2020年第3期205-208,共4页Medicine and Pharmacy of Yunnan

基　　金：国家自然基金项目(81460268)。

摘　　要：目的制备携hVEGF165表达载体阳离子脂质微泡并测定其与hVEGF165表达载体的结合率。方法制备携hVEGF165表达载体阳离子脂质微泡,测定其粒径大小、浓度、Zeta电位;测定与hVEGF165表达载体的结合率。结果阳离子脂质微泡90%粒径范围:1μm-3μm,平均粒径:(1.31±0.23)μm,平均浓度:(3.17±0.16)×108/mL,Zeta电位:(28.00±1.84)mV,与hVEGF165表达载体的最大结合率:(35.34±0.71)%。结论采用机械振荡法制备的阳离子脂质微泡,粒径分布良好,浓度、Zeta电位稳定,与hVEGF165表达载体结合率较高,是携带基因表达的良好载体系统。Objective To prepare cationic lipid microvesicles carrying hVEGF165 expression vector and determine its binding rate to hVEGF165 expression vector.Methods Cationic lipid microbubbles containing hVEGF165 expression vector were prepared and their particle size,concentration and Zeta potential were measured.The binding rate to hVEGF165 expression vector was determined.Results The particle size range of 90%cationic lipid microbubbles was 1μm-3μm,the average particle size was(1.31±0.23)μm,the average concentration was(3.17±0.16)×108/mL,the Zeta potential was(28.00±1.84)mV,and the maximum binding rate to hVEGF165 expression vector was(35.34±0.71)%.Conclusion The cationic lipid microbubbles prepared by mechanical oscillation method have good particle size distribution,stable concentration and Zeta potential,and high binding rate with hVEGF165 expression vector.

关 键 词：阳离子脂质微泡 超声造影剂 HVEGF165 

分 类 号：R34[医药卫生—基础医学]