20-羟基蜕皮甾酮对糖尿病大鼠脾损伤保护作用的研究  

Efficacy of 20-ydroxy-ecdysterone on injury to spleen in diabetic rats

作　　者：华春秀[1] Hua Chunxiu

机构地区：[1]南阳医学高等专科学校基础医学部,河南南阳473000

出　　处：《中医临床研究》2020年第10期1-5,共5页Clinical Journal Of Chinese Medicine

基　　金：河南省重点科技攻关项目(122102310103)。

摘　　要：目的:探讨20-羟基蜕皮甾酮(20E)对糖尿病大鼠脾脏功能的影响。方法:大鼠分为正常组、模型组、阳性对照组、20E低剂量处理组、20E中剂量处理组和20E高剂量处理组,正常组和模型组分别灌服生理盐水、阳性对照组动物给予50 mg/kg盐酸二甲双胍,20E低剂量处理组、20E中剂量处理组和20E高剂量处理组分别给予浓度为1 mg·kg^-1·d^-1、10 mg·kg^-1·d^-1和100 mg·kg^-1·d^-1的20E溶液。采用苏木精-伊红染色法(Hematoxylin-eosin Staining,HE)观察脾脏的形态学改变,测定血液相关生化指标,分析过氧化物歧化酶(Superoxide Dismutase,SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(Glutathione Peroxidase,GSH-Px)、谷胱甘肽还原酶(Glutathione Reductase,GR)、白介素(Interleukin,IL)-7和IL-10的表达。结果:与正常组相比,模型组大鼠脾脏明显损伤。与模型组相比,20E高剂量组SOD、CAT、GSH-Px和GR水平分别提高了78.63%(P<0.05)、51.76%(P<0.05)、2.95倍(P<0.01)和41.11%(P<0.05)。20E高剂量组IL-7和IL-10水平分别增加1.66倍(P<0.01)和1.21倍(P<0.01)。结论:20E对糖尿病大鼠脾脏具有保护作用,其机制可能与增强脾脏抗氧化能力和提高IL-7、IL-10表达有关。Objective:To explore the efficacy of 20-ydroxy-ecdysterone on injury to spleen in diabetic rats.Methods:Animals were divided into 6 groups,including normal group,model group,control group,low-dose 20 E group,middle-dose 20 E group and high-dose 20 E group.The normal group and model group were given normal saline.The control group took metformin hydrochloride with 50 mg/kg.Low-dose 20 E group,middle-dose 20 E group and high-dose 20 E group 20 E were given 20 E of different doses.Levels of biological parameters,anti-oxidative enzymes,interleukin-7(IL-7)and IL-10 were measured.Results:Compared with normal group,an obviously injury was observed in the model group.Compared with model group,SOD、CAT、GSH-Px and GR were increased to 78.63%(P<0.05),51.76%(P<0.05),2.95 times(P<0.01)and 41.11%(P<0.05)in the high-dose group,respectively.IL-7 and IL-10 were increased to 1.66 times(P<0.01)and 1.21 times(P<0.01)in the high-dose group,respectively.Conclusion:It is suggested that 20 E may play a protecting role for spleen injury in diabetic rats by enhancing the antioxidative ability and evaluating the IL-7 and IL-10 expression.

关 键 词：20-羟基蜕皮甾酮 糖尿病大鼠 脾脏损伤 保护作用 

分 类 号：R587.1[医药卫生—内分泌]