体外受精周期精子参数与胚胎发育动力学参数的相关性研究  

The study on the correlation between sperm parameters and embryo morphokinetics during in vitro fertilization

作　　者：王世凯[1] 谭卫红[1] 黄悦悦[1] 毛献宝[1] 李政达 张小慧[1] 韦娉嫔 刘庆友[2] 薛林涛[1] WANG Shi-kai;TAN Wei-hong;HUANG Yue-yue;MAO Xian-bao;LI Zheng-da;ZHANG Xiao-hui;WEI Pingpin;LIU Qing-you;XUE Lin-tao(Center of Reproductive Medicine and Genetic,People′s Hospital of Guangxi Zhuang Autonomous Region,Nanning,Guangxi 530021,China;State Key Laboratory for Conservation and Utilization of Subtropical Bioresources,Guangxi University,Nanning,Guangxi 530004,China)

机构地区：[1]广西壮族自治区人民医院生殖医学与遗传中心,南宁530021 [2]广西大学亚热带生物资源保护与利用国家重点实验室,南宁530004

出　　处：《中国优生与遗传杂志》2020年第2期250-254,共5页Chinese Journal of Birth Health & Heredity

基　　金：国家自然科学基金课题(31860638);广西卫生和计划生育委员会自筹经费科研课题(Z20170336);南宁市青秀区重点研发计划项目(2018025)。

摘　　要：目的探讨体外受精周期精子参数与胚胎发育动力学参数的相关性。方法回顾性分析2016年10月-2018年10月在我院进行Time-lapse监测培养的130个体外受精周期,取卵当日对用于受精的精子标本进行常规参数、精子动力学参数、精子DNA碎片率及顶体完整率检测,同时监测胚胎体外发育的动力学参数,分析精子参数和胚胎发育动力学参数之间的关系。结果①精子总活力(PN+PR)与tPNa和t5等动力学参数均呈显著负相关(P<0.05)。②精子的动力学参数LIN与tPNf呈显著正相关(P<0.05);WOB与tPNf和t2均呈显著正相关(P<0.05)。③精子DNA碎片率(SDF)和精子自发顶体反应率均与tPNa呈显著正相关(P<0.05);精子顶体完整率与tPNa呈显著负相关(P<0.05)。结论体外受精周期精子参数与胚胎发育动力学参数存在相关性,精子质量影响受精后合子原核出现时间。Objective:To explore the correlation between sperm parameters and embryo morphokinetics during in vitro fertilization.Methods:Totally 130 IVF treatment cycles using Time-lapse were analyzed retrospectively in our hospital from October 2016 to October 2018.On the day of oocyte collection,the routine parameters,sperm dynamics parameters,sperm DNA fragmentation rate and acrosome integrity rate of sperm samples for fertilization were measured.At the same time,the embryo morphokinetics were monitored,and the relationship between sperm parameters and embryo morphokinetics was analyzed.Results:①There were negative significant correlations between PR+NP with tPNa and t5(P<0.05).②There were positive significant correlations between LIN with tPNf(P<0.05),the WOB was positively correlated with tPNf and t2(P<0.05).③There was positive significant correlations between Sperm DNA fragmentation rate and sperm spontaneous acrosome reaction rate with tPNa(P<0.05);acrosome integrity rate was negatively correlated with tPNa(P<0.05).Conclusion:There is a correlation between sperm parameters and embryo morphokinetics during in vitro fertilization,Sperm quality affects the time of pronuclei appearance after fertilization.

关 键 词：精子参数 胚胎动力学参数 TIME-LAPSE 精子功能 

分 类 号：R321-33[医药卫生—人体解剖和组织胚胎学]