前列腺小体外泄蛋白在男性不育诊断治疗中的应用观察  

Application of prostatic exosomal protein in diagnosis and treatment of male infertility

作　　者：杨洋[1] 孟洁 白万凯 蔡蛟龙 王树玉[1] YANG Yang;MENG Jie;BAI Wan-kai;CAI Jiao-long;WANG Shu-yu(Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing 100026;Onco Biomedical Technology(Suzhou)Co.,Ltd,Taicang Jiangsu 214500)

机构地区：[1]首都医科大学附属北京妇产医院,北京100026 [2]昂科生物医学技术(苏州)有限公司,江苏太仓215400

出　　处：《中国优生与遗传杂志》2020年第2期258-259,共2页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨和观察前列腺小体外泄蛋白(prostatic exosomal protein,PSEP)指标在男性不育诊断治疗中的应用价值。方法随机选择我院门诊60例男性不育患者,作为实验组,收集尿液标本后并收取其精液标本。同时选取180例体检男性尿液作为对照组。采用酶联免疫法检测实验组和对照组尿样中的PSEP浓度。(1)分析实验组和对照组PSEP浓度水平的差异;(2)在实验组中,分析PSEP浓度和精液量、精子浓度、精子活性、精子正常形态率、酸性磷酸酶、精浆锌之间的相关性。结果经测试,实验组PSEP阳性率(62.5%)显著高于对照组PSEP阳性率(17.4%)(P<0.001)。在实验组中,PSEP浓度和b级活性精子数量呈显著负相关(P=0.039,r=-0.277),和精子PR(a+b)指标也较明显负相关(P=0.054,r=-0.259);PSEP浓度和精液量呈正相关(P=0.028,r=0.293),PSEP浓度和其他精液指标暂未发现相关性。结论尿液PSEP在男性不育患者中有较高的阳性率,且PSEP浓度和精子活性呈现负相关;尿液PSEP指标对男性不育诊断中的病因研究分析和指导治疗具有较大临床意义,应继续扩大样本量进一步研究探讨。Objective:To investigate and observe the application value of prostatic exosomal protein(PSEP)in the diagnosis and treatment of male infertility.Methods:60 male infertility patients in our hospital were selected.The urine was collected before the operation as the experimental group.180 male urine samples were selected as the control group.The semen indicators corresponding to each patient in the experimental group were recorded.The concentration of PSEP in the urine samples of the experimental group and the control group was determined by Enzyme-linked immunosorbent assay.(1)Analyze the difference of PSEP concentration levels between the experimental group and the control group;(2)Analyze the PSEP concentration and semen volume,sperm concentration,sperm activity,normal sperm morphology rate,acid phosphatase,and seminal plasma zinc in the experimental group.Results:The positive rate of PSEP in the experimental group(62.5%)was significantly higher than that in the control group(17.4%)(P<0.001).In the experimental group,there was a significant negative correlation between PSEP concentration and b-level active sperm count(P=0.039,r=-0.277),and negative correlation with sperm PR index(P=0.054,r=-0.259);PSEP concentration There was a positive correlation with semen volume(P=0.028,r=0.293),and no correlation was found between PSEP concentration and other semen indicators.Conclusion:Prostate small in vitro protein as an auxiliary index for the diagnosis of prostate disease,also has a high positive rate in male infertility patients,and PSEP concentration and sperm activity are negatively correlated,so the urine PSEP index in the diagnosis of male infertility etiological research analysis and guided treatment have superior significance,and further expansion of sample size will be further studied.

关 键 词：前列腺小体外泄蛋白 前列腺疾病 男性不育 精液分析 

分 类 号：R698.2[医药卫生—泌尿科学]