四季报春长短花柱生理特性差异研究  

Study on the difference of physiological characteristics of Primula obconica long and short style

作　　者：李娟 朱书愿 李孟悦 张晓曼[1] Li Juan;Zhu Shuyuan;Li Mengyue;Zhang Xiaoman(College of Landscape and Travel,Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北农业大学园林与旅游学院,河北保定071000

出　　处：《农业科学研究》2020年第1期39-42,67,共5页Journal of Agricultural Sciences

基　　金：河北省林业厅科学技术研究项目(1402450);河北省教育厅重点项目(ZD2016132)。

摘　　要：为研究四季报春长短花柱雌蕊各结构在不同时期生理成分的变化,以四季报春为试验材料,使用氮蓝四(NBT)光还原测定法、愈创木酚法、考马斯亮蓝法、分光光度计测定法分别测定四季报春长花柱、短花柱不同花期SOD活性、POD活性、可溶性蛋白含量、过氧化氢酶含量。结果表明:长花柱在花蕾期和衰败期雌蕊各结构SOD活性均低于短花柱,但在盛花期长花柱雌蕊各结构比短花柱SOD活性高;长花柱在不同时期柱头和花柱POD活性均低于短花柱,长花柱花蕾期活性最高,到衰败期后长花柱柱头POD活性与花蕾期接近,长花柱子房内POD活性在衰败期达到最高;长花柱柱头在各时期可溶性蛋白含量最高,长花柱、短花柱花柱在衰败期CAT活性最高,其他时期长花柱、短花柱雌蕊各结构可溶性蛋白含量、CAT活性均相差不大。In order to study the changes of physiological components in the pistils of Primula obconica, the test was based on Primula obconica, the nitrogen blue tetramine(NBT) photoreduction method, the guaiacol method, and the Coomassie blue method and spectrophotometer method were used to determine the SOD activity, POD activity, soluble protein content and catalase content in different periods of Primula obconica. The conclusions are as follows: the SOD activity in the flower bud stage and the decay stage of the long style column is lower than that of the short style, but in the full flowering stage,the SOD activity of the long style is higher than short style;The POD activity of the stigma and style of the long style was lower than that of the short style, and the activity of the long flower column was the highest. After the decay period, the POD activity of the long stigma was close to that of the flower bud, and the POD activity in the ovary of the long flower column reached the highest in the decay period. Except for the long-column stigma and the highest activity of CAT in the decay period, the soluble protein content and CAT activity of the pistil of the long and short stigma were similar.

关 键 词：四季报春 长花柱、短花柱 柱头 花柱 子房 

分 类 号：S682.1[农业科学—观赏园艺]