MGRN1对小鼠睾丸支持细胞自噬的影响  

Influence of MGRN1 on the autophagy of Sertoli cells in mice

作　　者：朱元[1] 罗勇[2] 卢海源 刘发英[2] 汪利群[1] ZHU Yuan;LUO Yong;LU Hai-yuan;LIU Fa-ying;WANG Li-qun(Department of Reproductive Health,Jiangxi Hospital for Women and Children,Nanchang,Jiangxi 330006,China;Central Laboratory,Jiangxi Hospital for Women and Children,Nanchang,Jiangxi 330006,China;Research Institute of Clinical Pharmacology,Xiangya Hospital,Central South University,Changsha,Hunan 410000,China)

机构地区：[1]江西省妇幼保健院生殖健康科,江西南昌330006 [2]江西省妇幼保健院中心实验室,江西南昌330006 [3]中南大学湘雅医院检验科,湖南长沙410000

出　　处：《中华男科学杂志》2020年第4期297-302,共6页National Journal of Andrology

基　　金：国家自然科学基金(81660265);江西省自然科学基金(20181BAB205015);江西省卫生计生委科技计划(20181121,20175430,20161111)。

摘　　要：目的:研究MGRN1对小鼠睾丸支持细胞自噬的影响。方法:体外培养小鼠睾丸支持细胞株(TM4细胞)利用RNAi下调TM4细胞中MGRN1的表达,然后利用Western印迹(WB)检测自噬相关蛋白(LC3-II/I、ATG-5和ATG-7)的表达,免疫荧光及电镜检测MGRN1下调后TM4细胞的自噬小体。结果:MGRN1下调后,WB发现TM4细胞中自噬相关蛋白(LC3-II/I、ATG-5和ATG-7)表达增加,荧光显微镜可观察到TM4细胞中自噬小体增加,与对照组相比,差异具有统计学意义(P<0.05),电镜可观察到TM4细胞中自噬小体增加。结论:MGRN1影响睾丸支持细胞的自噬,具体分子机制有待进一步研究。Objective: To study the effect of mahogunin ring finger-1(MGRN1) on the autophagy of Sertoli cells in mice. Methods: Using RNA interference, we down-regulated the expression of MGRN1 in the mouse TM4 Sertoli cells cultured in vitro, determined the expressions of the autophagy-related proteins LC3-II/I, ATG-5 and ATG-7 by Western blot, and detected the autophagosomes in the TM4 cells by immunofluorescence and electron microscopy. Results: Western blot showed increased expressions of LC3-II/I, ATG-5 and ATG-7 in the mouse TM4 Sertoli cells after knockdown of MGRN1. Fluorescence microscopy revealed significantly more autophagosomes in the TM4 cells than in the control group(P < 0.05). Conclusion: MGRN1 affects the autophagy of mouse Sertoli cells, and its specific molecular mechanism needs to be further studied.

关 键 词：MGRN1 小鼠睾丸支持细胞 自噬 

分 类 号：R698+.2[医药卫生—泌尿科学]