Molecular cloning and comparative analysis of transcripts encoding chemosensory proteins from two plant bugs, Lygus lineolaris and Lygus hesperus  

作　　者：J.Joe Hull Omaththage P.Perera Mei-Xian Wang 

机构地区：[1]USDA-ARS Arid Land Agricultural Research Center,Maricopa,Arizona,USA [2]USDA-ARS,Southern Insect Management Research Unit,Stoneville,Mississippi,USA [3]College of Animal Sciences,Zhejiang University,Hangzhou,China

出　　处：《Insect Science》2020年第3期404-424,共21页昆虫科学（英文版）

摘　　要：Chemosensory proteins(CSPs)are soluble carrier proteins typically characterized by a six‐helix bundle structure joined by two disulfide bridges and a conserved Cys spacing pattern(C1‐X6‐8‐C2‐X16‐21‐C3‐X2‐C4).CSPs are functionally diverse with reported roles in chemosensation,immunity,development,and resistance.To expand our molecular understanding of CSP function in plant bugs,we used recently developed transcriptomic resources for Lygus lineolaris and Lygus hesperus to identify 17 and 14 CSP‐like sequences,respectively.The Lygus CSPs are orthologous and share significant sequence identity with previously annotated CSPs.Three of the CSPs are predicted to deviate from the typical CSP structure with either five or seven helical segments rather than six.The seven helix CSP is further differentiated by an atypical C3‐X3‐C4 Cys spacing motif.Reverse transcriptase PCR‐based profiling of CSP transcript abundance in adult L.lineolaris tissues revealed broad expression for most of the CSPs with antenna specific expression limited to a subset of the CSPs.Comparative sequence analyses and homology modeling suggest that variations in the amino acids that comprise the Lygus CSP binding pockets affect the size and nature of the ligands accommodated.

关 键 词：CHEMOSENSATION chemosensory protein homology modeling Lygus plant bug mired TRANSCRIPTOME 

分 类 号：Q96[生物学—昆虫学]