改良一步酶消化法培养人头皮毛乳头细胞  

Improving the one-step collagenase treatment for more efficient human dermal papilla cells culture

作　　者：丁琦[1,2] 周蓝波 刘静静 徐宇轩 孙蔚凌 范卫新[2] DING Qi;ZHOU Lan-bo;LIU Jing-jing;XU Yu-xuan;SUN Wei-ling;FAN Wei-xin(Department of Dermatology,Affiliated Hospital of Nantong University,Nantong 226001,China)

机构地区：[1]南通大学附属医院皮肤科,江苏南通226001 [2]南京医科大学第一附属医院皮肤科,江苏南京210029

出　　处：《临床皮肤科杂志》2020年第5期267-270,共4页Journal of Clinical Dermatology

基　　金：国家自然科学基金(81972954)资助项目。

摘　　要：目的:通过改良一步酶消化法,快速便捷地培养人头皮毛乳头细胞。方法:采用一步酶消化法培养人头皮毛乳头细胞,并鉴定培养的人头皮毛乳头细胞。结果:通过镜下观察,发现一步酶消化法与改良二步酶消化法获得人头皮毛乳头细胞的有效率相当;相比传统方法,耗时更少,操作更便捷。培养的毛乳头细胞冻存复苏效果佳。结论:一步酶消化法可有效及高效地培养人头部毛乳头细胞。Objective: To improve the one-step enzyme digestion method, making the in vitro isolation and culture of human scalp dermal papilla cells more rapid and efficient. Methods: The improved one-step enzyme digestion method was employed to culture human dermal papilla cells which were then identified by alpha-SMA immunofluorescent staining. Results:Microscopic observation revealed that the efficiency of obtaining human dermal papilla cells by the improved one-stop enzyme digestion method was comparable to that of the improved two-step method. Compared with the conventional methods, human workload and operating time were reduced significantly. The cultured dermal papilla cells had a good recovery after cryopreservation. Conclusion: The improved one-step enzyme digestion method can culture human dermal papilla cells efficiently.

关 键 词：一步酶消化法 人毛乳头细胞 培养 

分 类 号：R758.71[医药卫生—皮肤病学与性病学]