尿路致病性大肠埃希菌的耐药和毒力全基因组分析  

Genome-wide analysis of drug resistance and virulence of uropathogenic Escherichia coli

作　　者：杨东靖[1] 苏旭[1] 吕莉琨[1] 张维[1] 王源[1] 于爱萍 李晓燕[1] Yang Dongjing;Su Xu;Lyu Likun;Zhang Wei;Wang Yuan;Yu Aiping;Li Xiaoyan(Tianjin Centers for Disease Control and Prevention,Tianjin 300011,China)

机构地区：[1]天津市疾病预防控制中心,300011

出　　处：《中华微生物学和免疫学杂志》2020年第4期269-275,共7页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金(81000707)。

摘　　要：目的:从基因组水平上探讨尿路致病性大肠埃希菌UPEC132株的耐药性和致病机制。方法:采用MIC法测定菌株UPEC132对16种抗菌药物的敏感性,多位点序列分型(MLST)方法对其分型,利用三代测序平台对其进行全基因组测序和组装,并用Prodigal软件预测和数据库筛选进行耐药和毒力基因功能注释,然后收集GenBank上23株UPEC菌株的染色体基因组序列,利用RAxML软件对UPEC132进行系统进化分析并构建系统进化树。结果:UPEC132菌株对氨苄青霉素、氨苄青霉素/舒巴坦、四环素、红霉素、氯霉素、头孢唑林耐药,其MLST分型为ST10522型。该株全基因组包括一条完整的基因组(染色体)序列及两条质粒序列,染色体、质粒P1和P2的序列大小分别为5234468 bp、117139 bp和101356 bp,鸟嘌呤-胞嘧啶(GC)含量分别为50.48%、49.05%和54.04%。该株染色体、质粒P1和P2分别有4856、140和116个基因。其耐药基因多位于染色体,主要为耐多药外排泵基因簇,P2仅携带β-内酰胺酶基因blaTEM-1和四环素耐药基因tetG。UPEC132毒力基因也位于染色体,主要为菌毛黏附素9种、铁摄取系统5种和分泌性毒素3种。Afa菌毛和Ⅳ型菌毛基因簇分别位于质粒P1和P2上。系统进化树分析显示UPEC132与23株UPEC均不在同一分支。结论:UPEC132株的ST10522型为国内外首次报道的大肠埃希菌新ST型,其全基因组遗传信息被全面揭示,耐药性和致病性与其携带的多种耐药基因和毒力基因相关。Objective To investigate the drug resistance and pathogenic mechanism of a uropathogenic Escherichia coli(UPEC)strain UPEC132 at the genome-wide level.Methods The susceptibility of UPEC132 strain to 16 antimicrobial agents was determined by minimum inhibitory concentration(MIC)assay.The UPEC132 strain was genotyped by multilocus sequence typing(MLST).The three-generation sequencing platform was used to sequence and assemble the whole genome of the UPEC132 strain.Drug resistance and virulence gene function annotations were predicted by Prodigal software and screened by using genome database.Genome sequences of the UPEC132 strain and 23 other UPEC strains collected from GenBank were phylogenetically analyzed,and a phylogenetic tree was constructed by RAxML software.Results The UPEC132 strain was resistant to ampicillin,ampicillin/sulbactam,tetracycline,erythromycin,chloramphenicol and cefazolin.Its genotype was ST10522 by MLST.The whole genome of the UPEC132 strain included one complete genome(chromosome)and two plasmid sequences.The sequence sizes of the chromosome and plasmids 1 and 2 were 5234468 bp,117139 bp and 101356 bp,and the guanine-cytosine(GC)content was 50.48%,49.05%,and 54.04%,respectively.There were 4856,140 and 116 genes annotated in the chromosome and plasmids 1 and 2,respectively.Drug resistance genes were mainly distributed in the chromosomal genome,mainly including the multidrug resistance efflux pump gene clusters.Only blaTEM-1 and tetG genes were carried in the plasmid 2.Virulence genes were also mainly distributed in the chromosome genome,including nine pilus adhesins,five iron uptake systems and three secretory toxins.Gene clusters encoding Afa and typeⅣfimbriae were located on plasmids 1 and 2,respectively.The phylogenetic tree showed that the UPEC132 strain was not in the same branch with either of the 23 UPEC strains.Conclusions The UPEC132 strain belonged to ST10522,which was a newly named ST type of Escherichia coli and first reported at home and abroad.The genome-wide genetic informat

关 键 词：尿路致病性大肠埃希菌 多位点序列分型 耐药性 毒力 全基因组 

分 类 号：R4[医药卫生—临床医学] R6