转录因子Blimp-1在原发性干燥综合征中的表达和作用  

Expression and role of transcription factor Blimp-1 in primary Sjögren′s syndrome

作　　者：吴若兰 郑旭 王怡平[2] 李向培 汪国生 陶金辉 厉小梅 Wu Ruolan;Zheng Xu;Wang Yiping;Li Xiangpei;Wang Guosheng;Tao Jinhui;Li Xiaomei(Department of Rheumatology and Immunology,Anhui Provincial Hospital Affiliated to Anhui Medical University,Hefei 230001,China;Centre for Transplantation and Renal Research,Westmead Institute for Medical Research,the University of Sydney,Sydney NSW 2006,Australia)

机构地区：[1]安徽医科大学附属省立医院风湿免疫科,合肥230001 [2]悉尼大学医学院Westmead研究所移植和肾脏病研究中心,NSW 2006

出　　处：《中华微生物学和免疫学杂志》2020年第4期276-282,共7页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金面上项目(81871271);安徽省重点研究与开发计划(1804b06020354)。

摘　　要：目的:探讨B淋巴细胞诱导成熟蛋白1(B lymphocyte induced maturation protein 1,Blimp-1)在原发性干燥综合征(pSS)外周血CD4^+T和CD19^+B细胞及唇腺组织的表达及其与临床指标的相关性。方法:采用RT-PCR技术检测外周血CD4^+T和CD19+B细胞及唇腺中PRDM1 mRNA表达水平。免疫组化观察Blimp-1蛋白在唇腺组织中的表达。评估PRDM1 mRNA与临床指标之间的相关性。结果:pSS组CD4+T和CD19^+B细胞中PRDM1 mRNA表达水平高于健康对照(healthy control,HC)组(P<0.01)。根据欧洲抗风湿联盟干燥综合征疾病活动指数(European League Against Rheumatism Sjogren′s Syndrome Disease Activity Index,ESSDAI)将pSS患者分为活动组(ESSDAI≥5)和非活动组(ESSDAI<5),活动组CD4+T和CD19+B细胞中PRDM1 mRNA表达水平高于非活动组(P<0.05)。pSS患者唇腺腺泡、导管周围及生发中心可见Blimp-1阳性聚集,唇腺PRDM1 mRNA表达水平与淋巴细胞浸润度呈正相关(r=0.781,P<0.001)。结论:Blimp-1高表达于pSS患者,可能影响pSS疾病活动,在pSS治疗中也具有一定的临床意义。Objective To investigate the expression of B lymphocyte induced maturation protein 1(Blimp-1)in peripheral blood CD4+T cells,CD19+B cells and labial glands of patients with primary Sjögren′s syndrome(pSS)and the correlation of Blimp-1 with clinical features.Methods Expression of PRDM1 at mRNA level in CD4^+T cells,CD19+B cells and labial gland tissues were detected by RT-PCR.Immunohistochemistry(IHC)was used to observe the distribution of Blimp-1.Correlation of PRDM1 expression at mRNA level with clinical indicators was analyzed.Results PRDM1 expression at mRNA level in CD4^+T and CD19^+B cells were significantly higher in pSS group than in healthy control(HC)group(P<0.01).Based on European League Against Rheumatism Sjögren′s Syndrome Disease Activity Index(ESSDAI),the patients were classified into two groups:active group(ESSDAI≥5)and inactive group(ESSDAI<5).PRDM1 expression at mRNA level in CD4+T and CD19+B cells were also higher in the active group than in inactive group(P<0.05).Blimp-1 protein accumulated around the acinus and duct of labial gland and in the germinal center in pSS patients.PRDM1 expression at mRNA level in labial gland tissues of pSS patients was positively correlated with lymphocyte infiltration(r=0.781,P<0.001).Conclusions pSS displayed high expression of Blimp-1.Blimp-1 might affect pSS disease activity and have clinical significance in pSS treatment.

关 键 词：原发性干燥综合征 慢性自身免疫病 BLIMP-1 B细胞 T细胞 

分 类 号：R593[医药卫生—内科学]