PD-1在类风湿关节炎外周血T淋巴细胞亚群表达的临床意义  

Clinical significance of PD-1 expression in peripheral blood T lymphocyte subsets of rheumatoid arthritis

作　　者：段厚全 叶小英[1,2] 葛彦 居颂光[1] DUAN Houquan;YE Xiaoying;GE Yan;JU Songguang(Department of Immunolgy,School of Basic Medicine and Life Science,Medical College of Soochow University,Suzhou,Jiangsu 215123,China;Department of Clinical Laboratory,Kunshan Hospital Affiliated to Jiangsu University,Kunshan,Jiangsu 215300,China)

机构地区：[1]苏州大学医学部基础医学与生命科学学院免疫学系,江苏苏州215123 [2]江苏大学附属昆山医院检验科,江苏昆山215300

出　　处：《国际检验医学杂志》2020年第11期1300-1305,共6页International Journal of Laboratory Medicine

基　　金：国家自然科学基金项目(31370887)。

摘　　要：目的检测类风湿关节炎(RA)患者外周血中的程序性死亡配体-1(PD-1)水平,分析其与常用临床实验室检测指标的相关性,并探讨其表达的临床意义。方法(1)收集RA患者及体检健康者的外周血标本,采用流式细胞术检测其PD-1水平;(2)记录RA的常用临床检测指标及其阳性率,并与PD-1进行相关分析与比较;(3)跟踪同一患者并观察其不同时期PD-1水平的变化。结果(1)在RA外周血中CD4+PD-1+T%和CD8+PD-1+T%水平活动期高于缓解期和健康对照组,差异均有统计学意义(均P<0.01);缓解期高于健康对照组,差异有统计学意义(P<0.05);(2)在RA外周血中其PD-1与C反应蛋白(CRP)和红细胞沉降率呈正相关关系(P<0.01)与抗环瓜氨酸肽抗体(抗CCP抗体)无明显相关性(P>0.05),且PD-1的阳性表达率高于CRP和红细胞沉降率,差异有统计学意义(P<0.01),而与抗CCP抗体阳性率比较,差异无统计学意义(P>0.05);(3)同一患者其外周血PD-1水平活动期明显高于缓解期,差异有统计学意义(P<0.01)。结论RA患者外周血PD-1水平高表达,并且PD-1与疾病严重程度密切相关;PD-1的阳性表达率高,并且与CRP、红细胞沉降率等指标密切相关,PD-1作为新的临床检测指标用于RA有一定的应用前景。Objective To detect programmed death ligand-1(PD-1)in peripheral blood of patients with rheumatoid arthritis(RA),analyze its correlation with commonly used clinical laboratory testing indicators,and explore the clinical significance of its expression.Methods The peripheral blood samples of RA patients and healthy people were collected,and their PD-1 was detected by flow cytometry.The common clinical detection indexes and their positive rates of RA were recoreded,and make correlation analysis and comparison with PD-1.The same patient was tracked and the changes of PD-1 in different periods were observed.Results The activity of CD4+PD-1+T%and CD8+PD-1+T%in the peripheral blood of RA was significantly higher than that in the remission period and the healthy control group(P<0.01).In the peripheral blood of RA,PD-1 was positively correlated with CRP and erythrocyte sedimentation rate(P<0.01),but not with anti CCP antibody(P>0.05),and the positive expression rate of PD-1 was higher than that of CRP and erythrocyte sedimentation rate(P<0.01),but not with anti CCP antibody(P>0.05).The activity period of PD-1 in peripheral blood of the same patient was significantly higher than that in remission period(P<0.01).Conclusion PD-1 is highly expressed in the peripheral blood of RA patients,and PD-1 is closely related to the severity of the disease.The positive expression rate of PD-1 is high,and it is closely related to CRP,erythrocyte sedimentation rate and other indicators.PD-1 as a new clinical detection indicator has a certain application prospect in RA.

关 键 词：类风湿关节炎 程序性死亡配体-1 检测指标 

分 类 号：R593.22[医药卫生—内科学]