大豆种荚应答镰刀菌侵染的叶绿素荧光成像规律  

Imaging rules in chlorophyll fluorescence of soybean pods in response to Fusarium verticillioides

作　　者：龙希洋 肖新力 张启辉 杨文钰[1] 刘江[1,2] 张静[1,3] LONG Xiyang;XIAO Xinli;ZHANG Qihui;YANG Wenyu;LIU Jiang;ZHANG Jing(Key Laboratory of Crop Ecophysiology and Farming System in Southwest,Ministry of Agriculture,Sichuan Agricultural University,Chengdu 611130,Sichuan,China;Institute of Ecological Agriculture,Sichuan Agricultural University,Chengdu 611130,Sichuan,China;College of Horticulture,Sichuan Agricultural University,Chengdu 611130,Sichuan,China)

机构地区：[1]四川农业大学农业部西南作物生理生态与耕作重点实验室,四川成都611130 [2]四川农业大学生态农业研究所,四川成都611130 [3]四川农业大学园艺学院,四川成都611130

出　　处：《浙江农林大学学报》2020年第3期540-546,共7页Journal of Zhejiang A&F University

基　　金：“十三五”国家重点研发计划重点专项(2016YFD0300209);国家自然科学基金资助项目(31971853);中国博士后科学基金资助项目(2017T100707)。

摘　　要：【目的】种荚是大豆Glycine max防御霉菌侵染的第1道防线,部分大豆品种种荚具有极佳的田间霉变抗性,本研究目的在于寻找与豆荚霉变抗性相关的快速鉴定指标。【方法】采用叶绿素荧光成像技术,以高抗大豆‘QWT15-2’、中抗大豆‘E1’和易感大豆‘E314’为研究对象,对离体种荚接种轮枝镰刀菌Fusarium verticillioides,监测其叶绿素荧光参数变化情况。【结果】大豆种荚接种霉菌24 h后,通过叶绿素荧光成像系统可清晰观察到豆荚表皮病斑,叶绿素荧光参数发生显著变化。霉菌侵染后0~5 d,大豆种荚初始荧光参数初始荧光(Fo)、最大荧光(Fm)、可变荧光(Fv)大幅降低,非光化学猝灭系数qN上升、QNP下降,最大光化学量子产量(Fv/Fm)、实际光化学效率(ΦPSⅡ)及电子传递速率(RET)均呈下降趋势。【结论】高抗大豆‘QWT15-2’维持了较健康的组织形态,各荧光参数表现稳定;中抗大豆‘E1’和感病大豆‘E314’受霉菌侵染影响,其表皮组织破坏严重、荧光参数变化幅度大;其中Fv/Fm、Fm、Fv、qN和QNP荧光参数对霉菌侵染响应敏感,可作为评价大豆种荚田间霉变抗性的鉴定指标。[Objective]The current study,in order to establish the index for the rapid identification of the resistance of soybean pods,is focused on three cultivars of cultivars,namely the high-resistant cultivar‘QWT15-2’,the medium-resistant cultivar‘E1’and the sensitive cultivar‘E314’.[Method]The pods were first inoculated with Fusarium verticillioides in vitro,and then the changes of chlorophyll fluorescence parameters in pods were monitored with the employment of chlorophyll fluorescence imaging technology.[Result]After 24 hours of inoculation,the epidermal lesions of the pods were clearly observed through the chlorophyll fluorescence imaging system,and there was a significant change in the fluorescence parameters.To be specific,0−5 days after mold infection,there was a significant decrease in the initial fluorescence parameters Fo,Fm,Fv of soybean pods,an increase in the non-photochemical quenching coefficient qN and a decrease in QNP,which was accompanied with an inclination of decline in the maximum photochemical efficiency Fv/Fm,actual photochemical efficiencyΦPSⅡand electron transport rate RET.[Conclusion]The high-resistant culivar‘QWT15-2’maintained a relatively healthy tissue with stable fluorescence parameters.The medium-resistant cultivar‘E1’and the sensitive cultivar‘E314’were affected by mold infection with severe epidermal tissue damage and significant change in the fluorescence parameters.Fluorescence parameters such as Fv/Fm,Fm,Fv,qN and QNP are sensitive to mold infection and can be used as an indicator to evaluate the resistance of soybean pods in the field.

关 键 词：大豆 种荚 轮枝镰刀菌 叶绿素荧光成像 抗性鉴定 

分 类 号：S332[农业科学—作物遗传育种]