苜蓿链霉菌内切β-N-乙酰氨基葡萄糖苷酶的克隆、表达及酶学性质  

Cloning,expression and characterization of a new endo-β-N-acetylglucosaminidase from Streptomyces alfalfae

作　　者：李玲聪 胡少锋 谷天燕 吕晨茵 刘妍池 刘华 顾金刚[2] 赵国刚 Lingcong Li;Shaofeng Hu;Tianyan Gu;Chenyin Lü;Yanchi Liu;Hua Liu;Jingang Gu;Guogang Zhao(College of Life Sciences,Hebei Agricultural University,Baoding 071000,Hebei,China;Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]河北农业大学生命科学学院,河北保定071000 [2]中国农业科学院农业资源与农业区划研究所,北京100081

出　　处：《生物工程学报》2020年第5期932-941,共10页Chinese Journal of Biotechnology

基　　金：国家重点研发计划(Nos.2017YFD0201100,2017YFD0200604);河北省留学人员科技项目(优秀类)(No.CP2015005015);农业部饲料生物技术重点实验室开放课题和河北农业大学启动资金(No.ZD201605)资助。

摘　　要：内切β-N-乙酰氨基葡萄糖苷酶广泛应用于糖生物学研究和工业生产。本研究从苜蓿链霉菌Streptomyces alfalfae ACCC 40021中克隆并原核表达了一个新的内切β-N-乙酰氨基葡萄糖苷酶,该酶最适反应温度为35℃,最适pH为6.0,具有良好的pH稳定性、温度稳定性和高比活(1×10^6 U/mg)的特性,可催化不同蛋白底物去糖基化,具有作为工具酶和生物催化剂的潜力。Endo-β-N-acetylglucosaminidase is used widely in the glycobiology studies and industries.In this study,a new endo-β-N-acetylglucosaminidase,designated as Endo SA,was cloned from Streptomyces alfalfae ACCC 40021 and expressed in Escherichia coli BL21(DE3).The purified recombinant Endo SA exhibited the maximum activity at 35ºC and pH 6.0,good thermo/pH stability and high specific activity(1.0×10^6 U/mg).It displayed deglycosylation activity towards different protein substrates.These good properties make EndoSA a potential tool enzyme and industrial biocatalyst.

关 键 词：内切β-N-乙酰氨基葡萄糖苷酶 去糖基化 苜蓿链霉菌 

分 类 号：Q936[生物学—微生物学]