硬毛粗盖孔菌子实体提取物对小鼠免疫功能及抗氧化能力的影响  

Effects of Funalia trogii Fruiting Body Extracts on Immune Function and Antioxidant Activity in Immunosuppressive Mice

作　　者：王洪发 刘颖[1,2] 王玉悦[1,2] 包海鹰 WANG Hongfa;LIU Ying;WANG Yuyue;BAO Haiying(Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University,College of Traditional Medicine,Jilin Agricultural University,Changchun,Jilin 130118,China)

机构地区：[1]吉林农业大学食药用菌教育部工程研究中心,吉林长春130118 [2]吉林农业大学中药材学院,吉林长春130118

出　　处：《食用菌学报》2020年第2期51-62,共12页Acta Edulis Fungi

基　　金：教育部长江学者和创新团队发展计划项目(IRT-15R25)。

摘　　要：通过注射环磷酰胺(80 mg·kg^-1)建立小鼠免疫抑制模型,分别灌胃给药小鼠硬毛粗盖孔菌(Funalia trogii)子实体石油醚、乙酸乙酯提取物(高、中、低剂量分别为20、10、5 mg·kg^-1)以及水提取物(高、中、低剂量分别为150、75、37.5 mg·kg^-1),通过测定脏器指数,观察HE染色切片和免疫组化切片,测定血清中免疫细胞因子、丙二醛、超氧化物歧化酶、溶菌酶、溶血素含量及总抗氧化能力,研究其对小鼠免疫功能及抗氧化能力的影响。结果表明:与模型组比较,水提取物高剂量组的胸腺、肝脏指数极显著增高,脾脏指数显著增高;乙酸乙酯提取物高剂量组的脾脏、肝脏指数极显著增高。水提取物高剂量组血清细胞因子IL-2、IL-4的分泌量极显著增加。水提取物高剂量组丙二醛的含量显著降低,且显著低于阳性组;水提取物高剂量组超氧化物歧化酶的含量显著增加;乙酸乙酯、水提取物高剂量组的总抗氧化能力极显著增强。水提取物各组的胸腺、脾脏细胞数量明显增加。研究表明硬毛粗盖孔菌子实体提取物能够增强小鼠的免疫功能及抗氧化能力,为其进一步开发利用提供参考。In this study, fruiting bodies of Funalia trogii were extracted in order by petroleum ether, ethyl acetate, and water, and then the resultant extract derived from each solvent was used to administer immunosuppressive mice induced by cyclophosphamide(CTX, 80 mg·kg^-1) at high, medium, and low doses, respectively. Mice in each treatment group were measured for organ indices, observed for organ sections by hematoxylin-eosin(HE) and immunohistochemical staining, and determined for serum cytokines, malondialdehyde(MDA), superoxide dismutase(SOD), lysozyme and serum hemolysin level.The results showed that mice administered with high dose water extract had highly significant(P<0.01) greater thymus index and liver index, and significant greater spleen index compared with mice in the CTX group. Mice administered with high dose ethyl acetate extract showed highly significant(P<0.01) greater spleen index and live index than mice in the CTX group. Excretion of IL-2 and IL-4 were significantly(P<0.01) increased in mice administered with high dose water extract compared with CTX treated mice. Compared with CTX treated mice, high dose water extract treated mice also had greater SOD levels. In contrast, their MDA levels were significantly lower than CTX treated mice and levamisole hydrochloride treated mice. Total antioxidant capacity was highly significantly(P<0.01) greater in high dose water extract treated mice and high dose ethyl acetate treated mice than in CTX treated mice.Water extract treated mice had a larger population of thymus and spleen cells than CTX treated mice. In summation, the results suggest that Funalia trogii can enhance immune function and antioxidant activity in vivo, thereby providing a basis for further development and utilization of it.

关 键 词：硬毛粗盖孔菌 免疫功能 抗氧化 免疫组化 

分 类 号：R285.5[医药卫生—中药学]