丹参川芎嗪注射液通过氧化应激反应对心肾综合征大鼠心肾损伤的影响  

Effect of Danshen and Ligustrazine Injection on cardiorenal injury in rats with cardiorenal syndrome through oxidative stress

作　　者：杨军[1] 许波[1] 蒲阳[1] 李熠 王华[2] 李蓉[3] 赵巍[4] YANG Jun;XU Bo;PU Yang;LI Yi;WANG Hua;LI Rong;ZHAO Wei(Department of cardiovascular medicine,Langzhong People's Hospital,Sichuan Province 637400,China;Department of Cardiology,West China Hospital,Sichuan University,Chengdu 610000,China;Department of Nephrology,Chengdu Integrative Hospital,Chengdu 610000,China;Department of Nephrology,First Teaching Hospital of Tianjin University of TCM,Tianjin 300000,China)

机构地区：[1]阆中市人民医院心血管内科,四川阆中637400 [2]四川大学华西医院心内科,四川成都610000 [3]成都市中西医结合医院肾内科,四川成都610000 [4]天津中医药大学第一附属医院肾内科,天津300000

出　　处：《药物评价研究》2020年第5期847-852,共6页Drug Evaluation Research

摘　　要：目的探究丹参川芎嗪注射液对心肾综合征(CRS)大鼠心肾损伤及氧化应激反应的影响。方法按照随机数字表法将大鼠分为假手术组、模型组、卡托普利(阳性药,13.0mg/kg)组以及丹参川芎嗪低、中、高剂量(0.4、1.0、2.0 mL/kg)组,假手术组仅暴露肾脏,不切除右肾及结扎左肾分支动脉,其余组采用永久性结扎大鼠冠状动脉左前降支1周后行3/4肾脏切除方法建立大鼠急性CRS模型。苏木精-伊红(HE)染色观察心脏、肾脏组织病理变化;化学比色法检测各组大鼠血清血肌酐(Scr)、尿素氮(BUN)水平;ELISA法检测血清脑钠肽(BNP)水平以及心脏、肾脏组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-px)水平。结果与假手术组比较,模型组大鼠血清Scr、BUN、BNP水平以及心脏、肾脏组织中MDA含量均显著升高(P<0.05),心脏、肾脏组织中SOD、GSH-px活性显著降低(P<0.05),同时可见心肌细胞肥大、排列紊乱,部分肾小管扩张,肾小球增大、结构模糊,刷状缘脱落;与模型组比较,丹参川芎嗪低、中、高剂量组和卡托普利组大鼠血清Scr、BUN、BNP水平以及心脏、肾脏组织中MDA含量均显著降低(P<0.05),心脏、肾脏组织中SOD、GSH-px活性显著升高(P<0.05),同时心脏、肾脏组织病理损伤程度明显改善,并呈剂量相关性。结论丹参川芎嗪注射液可有效改善CRS诱导的心肾损伤,可能与氧化应激反应密切相关。Objective To investigate the effects of Danshen Chuanxiongqin Injection on cardiorenal injury and oxidative stress in rats with cardiorenal syndrome(CRS).Methods Rats were divided into sham operation group,model group,Danshen Chuanxiongqin Injection low,medium and high dose groups and captopril group according to random number table method.The acute CRS rat model was established by 3/4 nephrectomy at one week after permanent ligation of left anterior descending coronary artery in rats.Hematoxylin-eosin(HE)staining was used to observe the pathological changes of heart and kidney tissues.Serum Scr and BUN levels were detected by chemical colorimetry.Serum BNP level and SOD,GSH-px and MDA levels in heart and kidney tissues were detected by ELISA.Results Compared with sham-operated group,the levels of serum Scr,BUN and BNP and the content of MDA in heart and kidney tissues in model group were significantly increased(P<0.05),the activities of SOD and GSHpx in heart and kidney were significantly decreased(P<0.05).at the same time,cardiomyocyte hypertrophy,disordered arrangement,dilatation of some renal tubules,enlargement of glomerulus,blurred structure and brush-like edge shedding were observed.Compared with model group,the levels of serum Scr,BUN,BNP and the content of MDA in heart and kidney of rats in low,medium and high dose groups of Danshen Chuanxiongqin Injection and captopril groups were significantly lower(P<0.05),the activities of SOD and GSH-px in heart and kidney increased significantly(P<0.05),at the same time,the degree of pathological damage of heart and kidney was significantly improved,which were in dose-dependent manners.Conclusions Danshen Chuanxiongqin Injection can effectively improve CRS-induced heart and kidney injury,which may be closely related to oxidative stress.

关 键 词：丹参川芎嗪 心肾综合征 心肾损伤 氧化应激反应 

分 类 号：R965[医药卫生—药理学]