5种生物源农药对梨树腐烂病菌的室内毒力测定  

Toxicity Determination of Five Biological Source Pesticides Against Valsa mali var.pyri

作　　者：蒋晶晶 周昭旭[1,2] 杜蕙 JIANG Jingjing;ZHOU Zhaoxu;DU Hui(Institute of Plant Protection,Gansu Academy of Agricultural Sciences,Lanzhou Gansu 730070,China;Scientific Observing and Experimental Station of Crop Pests in Tianshui,Ministry of Agriculture,GanguGansu 741299,China)

机构地区：[1]甘肃省农业科学院植物保护研究所,甘肃兰州730070 [2]农业部天水作物有害生物科学观测实验站,甘肃甘谷741299

出　　处：《甘肃农业科技》2020年第5期25-29,共5页Gansu Agricultural Science and Technology

基　　金：国家重点研发计划(2018YFD0201404-4);公益性行业(农业)科研专项(201203035);甘肃省现代水果产业技术体系(GARS-SG-2);甘肃省科技支撑计划(1204NKCA099)。

摘　　要：采用生长速率法测定了5种生物源农药对梨树腐烂病菌菌丝生长的抑制效果。结果表明,除10%烟碱水剂外,其他4种生物源农药对梨树腐烂病菌均具有显著的抑制作用。当0.3%丁子香酚可溶液剂、0.3%苦参碱乳油、0.4%蛇床子素可溶液剂、0.5%小檗碱水剂质量浓度分别为0.50000、2.50000、2.50000、5.00000μg/mL时,其对梨树腐烂病菌菌丝生长的抑制率分别为94.9%、98.9%、94.5%和96.9%,EC50分别为0.0830、0.1449、0.2339、0.2687μg/mL;10%烟碱水剂的室内毒力最差,EC50为6344.6170μg/mL。综上所述,0.3%丁子香酚可溶液剂、0.3%苦参碱乳油、0.4%蛇床子素可溶液剂、0.5%小檗碱水剂对梨树腐烂病室内毒力较好。In this study,the inhibitory effects of five different biological source pesticide on mycelial growth by the method of mycelial growth rate.The results showed that except for 10%nicotine AS were the lowest toxic,four kinds of biological source pesticide had siginificant inhibitory effects on the growth of Valsa mali var.pyri.when the mass concentration of eugenol 0.3%SL,matrine 0.3%EC,osthole 0.4%SL,berberine 0.5%AS were 0.50000,2.50000,2.50000 and 5.00000μg/mL,the inhibitation rate on mycelial growth were 94.9%、98.9%,94.5%and 96.9%,and the EC50 values were 0.0830、0.1449、0.2339、0.2687μg/mL respectively.Nicotine 10%AS were the lowest toxic,EC50 values were 6344.6170μg/mL.In summary,among the five kinds of biological source pesticide,eugenol 0.3%SL,matrine 0.3%EC,osthole 0.4%SL,berberine 0.5%AS had good inhibitory effects.

关 键 词：梨树腐烂病菌 生物源农药 毒力测定 生长速率法 

分 类 号：S476[农业科学—农业昆虫与害虫防治]