Identification and Characterization of Tonoplast Sugar Transporter (TST) Gene Family in Cucumber  

作　　者：Weifeng Huang Bin Hu Jialin Liu Yong Zhou Shiqiang Liu 

机构地区：[1]National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research,Huazhong Agricultural University,Wuhan 430070,China [2]Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education,Jiangxi Agricultural University,Nanchang 330045,China [3]College of Bioscience and Bioengineering,Jiangxi Agricultural University,Nanchang 330045,China

出　　处：《Horticultural Plant Journal》2020年第3期145-157,共13页园艺学报（英文版）

基　　金：the Key Project of Science Foundation of Jiangxi Province(Grant Nos.20171ACB21025 and 20181ACB20012);the National Natural Science Foundation of China(Grant No.31760074).

摘　　要：Tonoplast sugar transporters(TSTs)play essential roles in regulating plant growth,development,and response to various biotic and abiotic stresses.In this study,a total of three TST genes were identified by a genome-wide analysis in cucumber.Phylogenetic analysis showed that TST proteins from cucumber and other plant species can be classified into five groups,and nearly all TST members in the same groups displayed similarmotif distributions,transmembrane(TM)domains,and gene structures.All of the three CsTST genes possess a number of development-,stress-,and hormone-related cis-elements in the promoter sequences.Meanwhile,qRT-PCR assays revealed that the CsTST1 was expressed in fruits,flowers,leaves,and other tissues,and its expression varied significantly under various abiotic stresses such as cold,salt,drought(PEG),and abscisic acid(ABA).Finally,functional analysis of CsTST1 in yeast revealed that it was able to complement the deficiency in galactose,mannose and sucrose transport.These results revealed that CsTST1 can act as a functional sugar transporter to play important roles in cucumber growth and response to abiotic stress probably through affecting carbohydrate distribution.

关 键 词：CUCUMBER Cucumis sativus L. TST Phylogenetic analysis Gene expression Abiotic stress Sugar transport 

分 类 号：S642.2[农业科学—蔬菜学]