Comparative Analysis of Leaf Trichomes, Epidermal Wax And Defense Enzymes Activities in Response to Puccinia horiana in Chrysanthemum and Ajania Species  

作　　者：Yinjie Wang Jun Zeng Xiaolong Xia Yang Xu Jing Sun Jing Gu Hainan Sun Haini Lei Fadi Chen Jiafu Jiang Weiming Fang Sumei Chen 

机构地区：[1]College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China

出　　处：《Horticultural Plant Journal》2020年第3期191-198,共8页园艺学报（英文版）

基　　金：supported by Jiangsu Agriculture Science and Technology Innovation Fund[Grant No.CX(18)2020];the National Natural Science Foundation of China(Grant Nos.31672192 and 31700620);the National Key Research and Development Program of China(Grant No.2018YFD1000402);the Fundamental Research Funds for the Central Universities(Grant No.KJQN201812).

摘　　要：White rust caused by Puccinia horiana is a destructive disease of chrysanthemum plants.To better understand the resistance mechanisms of composite species to this disease,the leaf cuticular traits,antioxidant and defensive enzymes activities of immune(Chrysanthemum makinoi var.wakasaense)and highly susceptible(Ajania shiwogiku var.kinokuniense)species were compared.Trichome density of two species was markedly different,negatively associated with plant resistance to P.horiana.Total wax load in C.makinoi var.wakasaense was two times more than that in A.shiwogiku var.kinokuniense.The wax composition in immune one was abundant in esters and primary alcohols.Superoxide dismutase(SOD,EC 1.15.1.1),peroxidase(POD,EC 1.11.1.7),polyphenoloxidase(PPO,EC 1.14.18.1 or EC 1.10.3.2)and phenylalanine ammonia lyase(PAL,EC 4.3.1.5)activitieswere investigated.In C.makinoi var.wakasaense,the activity of SOD and POD increased rapidly after inoculation,whichmight be non-host induced reactive oxygen species(ROS)activated antioxidant enzymes,however SOD and POD remained a low and steady level in the highly susceptible one after inoculation.Quick increase in PPO activities after inoculation was observed in both species,however it remained higher in C.makinoi var.wakasaense at the late period of inoculation.PAL in C.makinoi var.wakasaense was induced after pathogen inoculation,but not in A.shiwogiku var.kinokuniense,suggesting that these two enzymes might contribute to the resistance to P.horiana.

关 键 词：CHRYSANTHEMUM Puccinia horiana Resistance Leaf cuticular wax Enzyme activity 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]