基于增溶槲皮素的稳定纳米乳的制备  

Preparation of stable nanoemulsion based on solubilization of quercetin

作　　者：宁远兰 沈文 敖芬 NING Yuan-lan;SHEN Wen;AO Fen(School of Food and Biological Engineering,Shaanxi University of Science&Technology,Xi′an 710021,China)

机构地区：[1]陕西科技大学食品与生物工程学院,陕西西安710021

出　　处：《陕西科技大学学报》2020年第3期34-40,共7页Journal of Shaanxi University of Science & Technology

基　　金：陕西省咸阳市科技计划项目(2019k02-58);陕西省西安市未央区科技计划项目(201938);陕西省科技厅重点研发计划项目(2020SF-423);陕西科技大学博士科研启动基金项目(2019BJ-06)。

摘　　要：通过伪三元相图法筛选出空白纳米乳的组分,制备O/W型纳米乳,测定其结构类型、电导率、粘度、稳定性,对其微观形态及分布特征进行了表征,并考察纳米乳对难溶性药物槲皮素的增溶作用.结果表明,槲皮素纳米乳的组分为:乙酸乙酯/乳化剂OP-10/无水乙醇/纯净水;所制槲皮素纳米乳为澄清、透明的淡黄色液体,粘度0.024 Pa·s,电导率99.0μs/cm.通过透射电镜观察,纳米乳呈现球形形态;平均粒径(14.43±0.25)nm,多分散指数(PDI)0.157;Zeta电位1.25±0.45 mV,具有纳米级粒径,呈现稳定的正态粒径分布的O/W型乳剂特征.通过负载难溶性药物槲皮素,测得稳定的纳米乳体系中槲皮素的含量为354.84μg/mL,相较于槲皮素在水中的溶解度(0.17~7.7μg/mL),显著提高了槲皮素的水溶性.In this paper,the components of blank nanoemulsion were screened by pseudo-ternary phase diagram method to prepare O/W nanoemulsion.The structure,conductivity,viscosity and stability of the nanoemulsion were determined.The micromorphology and distribution characteristics were characterized and investigate the solubilization effect of nanoemulsion on the insoluble drug quercetin.The results show the components of quercetin nanoemulsion was ethyl acetate/emulsifier OP-10/anhydrous ethanol/pure water,the quercetin nanoemulsion prepared was clear and transparent yellowish liquid.Its viscosity was 0.024 Pa·s,conductivity was 99.0μs/cm.Through observation by transmission electron microscopy,the nanoemulsion exhibited spherical morphology;The prepared nanoemulsion had an average particle diameter of(14.43±0.25)nm and a polydispersity index(PDI)of 0.157;its Zeta potential was measured to be 1.25±0.45 mV.It has nano-sized particle size and exhibits a stable normal particle size distribution of O/W emulsion characteristics.By loading the insoluble drug quercetin,the content of quercetin in the stable nanoemulsion system was 354.84μg/mL.Compared with the solubility of quercetin in water(0.17~7.7μg/mL),the water solubility of quercetin was significantly improved.

关 键 词：纳米乳 槲皮素 伪三元相图 增溶 

分 类 号：O647.1[理学—物理化学]