应激性高血压大鼠血管组织内质网应激与血管紧张素Ⅱ受体1型的关系及其意义  

Relationship between endoplasmic reticulum stress and angiotensinⅡtype 1 receptor in vascular tissues of stress-induced hypertensive rats and its significance

作　　者：阿依西布·萨吾提 库热西·玉努斯 热孜亚·艾买提 纳菲沙·卡德尔[1] 吴桂霞[1] 钟莉[1] 希林古丽·吾守尔 麦尔哈巴·库尔班 Ayixibu Sawuti;Kurexi Yunusi;Reziya Aimaiti;Nafeisha Kadeer;WU Guixia;ZHONG Li;Xilinguli Wushouer;Maierhaba Kuerban(School of Preclinical Medicine,Xinjiang Medical University,Urumqi 830011,China;School of Uyghur Medicine,Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]新疆医科大学基础医学院,乌鲁木齐830011 [2]新疆医科大学维吾尔医学院,乌鲁木齐830011

出　　处：《新疆医科大学学报》2020年第5期537-541,共5页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区“十三五”重点学科建设项目(高原学科)。

摘　　要：目的初步探讨应激性高血压(Stress-induced hypertension,SIH)大鼠血管组织内质网应激(Endoplasmic Reticulum Stress,ERS)与血管紧张素Ⅱ受体1型(AngiotensinⅡtype 1 receptor,AT1R)的关系。方法通过复合应激建立SIH模型。对大鼠胸主动脉石蜡包埋组织进行HE染色,观察大鼠胸主动脉壁中层平滑肌层的形态学变化;用实时荧光定量PCR(RT-qPCR)方法﹑免疫组织化学(IHC)方法及Western印迹(WB)对大鼠胸主动脉组织进行grp78和at1r基因mRNA表达水平以及GRP78和AT1R的蛋白表达水平的检测。结果与正常组相比,SIH模型组中膜肌层血管平滑肌细胞(vescular smooth muscle cell,VSMC)肥大、动脉血管壁增厚、细胞排列较紊乱、弹性膜弯曲及紊乱;SIH模型组大鼠胸主动脉血管组织中at1r基因mRNA表达水平较正常组显著升高(P<0.05);与正常组相比,GRP78和AT1R在SIH模型组大鼠胸主动脉组织中高表达(P<0.05)。结论SIH模型组大鼠血管组织中有ERS的发生,ERS下游信号分子通过激活转录因子诱导血管组织中ATIR的高表达,从而引起血压的升高。Objective To investigate the relationship between Endoplasmic Reticulum Stress(ERS)and AT1 receptor(AT1R)in rats with stress-induced hypertension(SIH).Methods The SIH model was established by combined stress.The morphological changes of the middle smooth muscle layer of the thoracic aortic wall were observed by HE staining.The expression levels of grp78,at1r gene and GRP78,AT1R protein of thoracic aortic tissues were detected by real-time fluorescence quantitative assay PCR(RT-qPCR),immunohistochemistry(IHC)and western blotting(WB).Results Compared with the normal group,VSMC hypertrophy,arterial wall thickening,cell arrangement disorder,elastic membrane bending and disorder appeared in the SIH model group;the mRNA expression level of at1r gene in the thoracic aortic vascular tissues of rats in the SIH model group was significantly higher than that in the normal group(P<0.05);Compared with the normal group,GRP78 and AT1R were highly expressed in the thoracic aorta in the model group(P<0.05).Conclusion ERS may occur in the vascular tissues of rats in the SIH model group.The downstream signal molecules of ERS induce the high expression of ATIR in the vascular tissues by activating transcription factors,so as to cause the increase of blood pressure.

关 键 词：内质网应激 应激性高血压 GRP78 血管紧张素II受体1型 

分 类 号：R544.1[医药卫生—心血管疾病]