铅胁迫对垂柳生长和光合生理特性的影响  

Effects of lead stress on the growth and photosynthesis of Salix babylonica

作　　者：薛文秀 尚晓硕 蒋艺 邹金华[1,2] XUE Wenxiu;SHANG Xiaoshuo;JIANG Yi;ZOU Jinhua(College of Life Sciences,Tianjin Normal University,Tianjin 300387,China;Tianjin Key Laboratory of Animal and Plant Resistance,Tianjin Normal University,Tianjin 300387,China)

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]天津师范大学天津市动植物抗性重点实验室,天津300387

出　　处：《天津师范大学学报（自然科学版）》2020年第3期28-32,共5页Journal of Tianjin Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(31901184);天津师范大学博士基金资助项目(52XB1914).

摘　　要：为了探究铅胁迫下垂柳在生长和光合生理方面做出的响应,对垂柳枝条进行水培,施加不同浓度的Pb(NO3)2溶液(1、10、50和100μmol/L)处理28 d后,测量垂柳的株高、根长、光合色素含量以及叶绿素荧光参数等指标.结果发现,与对照组相比,1μmol/L的Pb(NO3)2溶液处理下,垂柳的株高和根长、光合色素含量、叶绿素荧光参数中的初始荧光(F0)、最大荧光(Fm)、PSⅡ最大光化学量子效率(Fv/Fm)、PSⅡ光化学能量转化的有效量子产量(Y(Ⅱ))、光化学淬灭系数(qP)均显著增加,非光化学淬灭系数(qN或NPQ)下降;10、50和100μmol/L的Pb(NO3)2溶液处理下,垂柳的生长指标、光合色素含量、叶绿素荧光参数的变化趋势则与之相反,且随着溶液浓度的增加,上述指标增加或降低的程度增大.与地上部分相比,垂柳根部生长受铅胁迫的抑制程度更大.总的来看,低浓度的铅胁迫会在一定程度上促进垂柳植株生长、光合色素生成以及光合作用,高浓度的铅胁迫则对垂柳生长和光合作用具有显著抑制作用.In order to explore the response of growth and photosynthetic characteristics in Salix babylonica to Pb stress,the shoots of Salix babylonica were cultivated in the solutions with different Pb(NO3)2 concentrations(1,10,50 and 100μmol/L).After 28 d cultivation,the shoot height,root length,photosynthetic pigment content,and chlorophyll fluorescence parameters were measured.The results showed that compared with the control,the shoot height,root length,photosynthetic pigment content,initial fluorescence(F0),the largest fluorescence(Fm),the maximum photochemical quantum efficiency of PSⅡ(Fv/Fm),effective quantum yield of photochemical energy conversion in PSⅡ(Y(Ⅱ)),photochemical quenching coefficient(qP)increased significantly,and nonphotochemical quenching coefficient(qN or NPQ)decreased after it was treated with 1μmol/L Pb(NO3)2 solution.When the shoots were treated with Pb(NO3)2 solutions with high concentrations(10,50 and100μmol/L),the change tendency of the growth indexes,the photosynthetic pigment content,and chlorophyll fluorescence parameters was on the contrary.And the change degree of these indexes increased with the increase of the concentration of Pb(NO3)2 solution.The inhibition of Pb on root was more serious than that on shoot.In conclusion,the growth,the photosynthetic pigment production and the photosynthesis of Salix babylonica were promoted at low concentration of Pb,and high concentrations of Pb had a significant inhibitory effect on Salix babylonica.

关 键 词：垂柳 铅胁迫 光合色素 叶绿素荧光参数 

分 类 号：Q945[生物学—植物学]