不同光质对‘秋红宝’葡萄试管苗生长的影响  

Effect of light quality on the growth of in vitro seedling of‘Qiuhongbao’grape

作　　者：杨忠义[1,2,3] 郭雯岩 李戌彦 董志刚 乔治军 纪薇 YANG Zhongyi;GUO Wenyan;LI Xuyan;DONG Zhigang;QIAO Zhijun;JIWei(College of Horticulture,Shanxi Agricultural University,Taigu 030801,Shanxi,China;Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture,Taiyuan 030031,Shanxi,China;Key Laboratory of Fruit Tree Germplasm Creation and Utilization,Taigu 030801,Shanxi,China;Institute of Crop Germplasm Resources,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,Shanxi,China)

机构地区：[1]山西农业大学园艺学院,山西太谷030801 [2]农业部黄土高原作物基因资源与种质创制重点实验室,太原030031 [3]果树种质创制和利用山西省重点实验室,山西太谷030801 [4]山西省农业科学院农作物品种资源研究所,太原030031

出　　处：《果树学报》2020年第6期838-847,共10页Journal of Fruit Science

基　　金：山西省高等学校青年科研人员培育计划(2019);国家农作物种质资源共享服务平台山西农作物种质资源平台(NICGR-2019-26);山西省科技厅重点研发项目(国际合作)(201703D421003)。

摘　　要：【目的】探索不同光质对‘秋红宝’葡萄试管苗生长及生理特性的影响。【方法】以‘秋红宝’葡萄试管苗为试材,设置7种不同光质处理,观测试管苗的株高、茎粗、叶片叶绿素含量及抗氧化酶活性等生理生化指标,并结合主成分分析和灰色关联分析筛选出最优光质。【结果】单白光与单红光处理的株高显著高于其他处理;单蓝光处理的植株根数和最长根长均达到各处理最大值,且叶片叶绿素含量均显著高于其他处理;红蓝光质4∶1处理的叶片SOD活性显著高于其他处理;POD与CAT活性在红蓝光质5∶1中最高。综合灰色关联分析得出最适光质排序为:红蓝光质5∶1>单蓝光>单白光>单红光>红蓝白光质3∶1∶1>红蓝光质4∶1>红蓝光质3∶1。【结论】红蓝5∶1光质可作为促进‘秋红宝’葡萄试管苗生长的最理想光质;影响不同光质下试管苗生长的主要指标有总叶绿素、最长根长、株高、叶长、SOD。【Objective】Light is one of the environmental factors affecting tissue culture for propagation.Light quality plays an important role in the growth,physiology and metabolite accumulation in in vitro seedlings.LED polychromatic light can promote the proliferation of in vitro seedlings.At present,the research of light quality on grape growth focuses mostly on vines grown in greenhouse,but there is little research on its application in tissue culture.Therefore,this study explored the effect of light quality on the growth and physiological characteristics of‘Qiuhongbao’grape seedlings grown in vitro【.Methods】Seven different light quality treatments were set up in this experiment.They were white light(W),red light(R),blue light(B),and combinations of red,blue or white light with different ratios(R∶B=3∶1,R∶B=4∶1,R∶B=5∶1,and R∶B∶W=3∶1∶1).The young stems of‘Qiuhongbao’grape were collected from the Horticultural Station of Shanxi Agricultural University.Healthy young stem segments of‘Qiuhongbao’were collected in the morning before dew dried.They were washed with water for 2 h to remove dust,cut it into 5 cm stem segments as explant,and surface disinfected in an ultra clean workbench.The specific disinfection method was 75%alcohol immersion for 30 s,washing with sterile water for 2-3 times,0.1%mercuric chloride immersion,and washing with sterile water for 5 times.The sterilized stem segments were cut into 2 cm single bud stem segments with both ends removed,and inoculated on MS+0.2 mg·L^-1 IBA+1.0 mg·L^-16-BA+0.2 mg·L^-1 NAA+30.0 g·L^-1 sucrose+7.0 g·L^-1 agar(pH 5.8-6.0).The culture environment temperature was(25±1)℃,and the photoperiod was 16/8 h(light/dark).10 days after inoculation,the survival rate,pollution rate and browning rate under different treatments were recorded.In each treatment,10 plantlets were randomly selected to measure seedling water content and their morphological indexes including plant height,stem diameter,leaf number,leaf length,leaf width,root number,m

关 键 词：‘秋红宝’葡萄 试管苗 红蓝光配比 抗氧化酶 

分 类 号：S663.1[农业科学—果树学]