电针对脑缺血再灌注损伤大鼠海马区细胞焦亡相关蛋白酶Caspase-1的影响  

Effects of Electroacupuncture on Pyroptosis-related Caspase-1 Expression in Hippocampus Region of Rats with Cerebral Ischemia-Reperfusion Injury

作　　者：许秀洪 周国平 董苗苗[2] 李曼玲 杨路 詹珠莲 罗梦[1] Xu Xiuhong;Zhou Guoping;Dong Miaomiao;Li Manling;Yang Lu;Zhan Zhulian;Luo Meng(Integrated Hospital of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China;School of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China)

机构地区：[1]南方医科大学中西医结合医院,广州510515 [2]南方医科大学中医药学院,广州510515

出　　处：《世界科学技术-中医药现代化》2020年第2期446-451,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：广东省中医药局科研项目(20161158):基于Caspase基因家族关键调控基因探讨电针抗脑缺血再灌注损伤的作用机制,负责人:许秀洪;国家自然科学基金委员会常规面上项目(81674048):电针对脑缺血再灌注后神经元损伤的关键调控基因Caspase-3、Caspase-1的干预作用研究,负责人:周国平。

摘　　要：目的观察电针疗法对局灶脑缺血再灌注(cerebral ischemia reperfusion,CIR)损伤大鼠海马区细胞焦亡相关蛋白酶半胱天冬酶-1(Caspase-1)的影响。方法Longa线栓法构建大鼠脑缺血再灌注损伤模型。选取30只雄性SD大鼠随机分为假手术组、模型组和电针治疗组,每组各10只。电针治疗组取足三里、三阴交、尺泽、合谷四穴进行针刺,每次20 min。Zea-Longa评分法评估各组大鼠神经功能。苏木精-伊红(hematoxylin-eosin,HE)染色和2,3,5-氯化三苯基四氮唑(triphenyltetrazolium chloride,TTC)染色法观察大鼠脑组织病理形态及脑梗死面积。实时荧光定量(Reverse Transcription-Polymerase Chain Reaction,RTPCR)、免疫印迹法(Western blot,WB)检测大鼠脑组织海马区焦亡相关蛋白酶Caspase-1mRNA和蛋白表达水平。结果与假手术组比较,模型组的神经行为学评分显著升高(P<0.01),出现明显的神经细胞损伤病理特征和白色脑梗死区,脑组织中Caspase-1的mRNA及蛋白水平显著上调(P<0.01)。与模型组相比,电针治疗组显著减轻大鼠神经功能缺损和病理损伤特征(P<0.01),白色区域脑梗死体积百分比明显缩小(P<0.01),海马区Caspase-1的转录水平和转录后水平显著降低(P<0.05)。结论电针疗法可能是通过拮抗Caspase-1介导的细胞焦亡的激活,保护脑缺血再灌注受损的神经细胞。Objective To study the effects of electroacupuncture(EA)on the expression of cell pyroptosis related Caspase-1 in hippocampus of focal cerebral ischemia-reperfusion(CI/R)damage in rats.Methods The CI/R injury model was induced by middle cerebral artery occlusion with intraluminal thread.A total of 30 male Sprague Dawley rats were randomly divided into a sham operation(sham)group,a model group and a EA group,with 10 rats in every group.EA(2/15 Hz,2 mA,20 min)was applied daily to Zusanli(ST 36),Sanyinjiao(SP 6),Chize(LU 5)and Hegu(LI 4).Then behavioral neurological assessment of all rats in each group was performed with Zea-Longa score method after CI/R injury.We also separately observed the neuronal damage and areas of cerebral infarct by HE staining and TTC staining method.The expression of Caspase-1 was measured by qPCR and western blot,respectively.Results Compared with the sham group,the scores of the model group were notably increased(P<0.01)and the brain tissue in model rats showed obviously neuronal injury and white infarction area,both up-regulated the mRNA and protein levels of Caspase-1(P<0.01).While compared with the model group,the neural deficit scores and neuron injury were relieved significantly and infarction area markedly shrank in EA group(P<0.01).Meanwhile,the expression of Caspase-1 at transcription and post-transcription levels in hippocampus of rats in the EA group were significantly decreased than those in the model group(P<0.05).Conclusion The protective effect of electroacupuncture therapy on rats with cerebral ischemia-reperfusion injury may inhibit the activation of Caspase-1 dependent pyroptosis.

关 键 词：电针 脑缺血再灌注损伤 细胞焦亡 CASPASE-1 

分 类 号：R246.1[医药卫生—针灸推拿学]