高效液相色谱法测定紫锥菊中7种酚酸含量及其聚类分析  

Determination of Seven Phenolic Acids in Echinacea purpurea by HPLC and the Cluster Analysis

作　　者：孙丽平 齐海艳 郑洪伟[3] 杜研 杨春娟 甘春丽 SUN Liping;QI Haiyan;ZHENG Hongwei;DU Yan;YANG Chunjuan;GAN Chunli(Department of Pharmaceutical Chemistry and Natural Pharmaceutical Chemistry,College of Pharmacy,Harbin Medical University,Harbin 150081,China;College of Traditional Chinese Medicine,Shenyang Pharmaceutical University,Shenyang 110016,China;China Institute of Water Resources and Hydropower Research,Beijing 100038,China)

机构地区：[1]哈尔滨医科大学药学院药物化学与天然药物化学教研室,哈尔滨150081 [2]沈阳药科大学中药学院,沈阳110016 [3]中国水利水电科学研究院,北京100038

出　　处：《医药导报》2020年第6期831-835,共5页Herald of Medicine

基　　金：黑龙江省卫生计生委科研课题(2018491);黑龙江省博士后资助经费资助(LBH-Z18105);哈尔滨医科大学创新创业项目(201810226065);黑龙江省高等教育协会“十三五”规划课题(16Q070)。

摘　　要：目的建立高效液相色谱(HPLC)法测定不同产地紫锥菊提取物中7种酚酸类成分的含量;层次聚类分析法(HCA)对其进行统计归类,为质量的一致性和合理应用提供依据。方法色谱柱为Inertsil C18色谱柱(250 mm×4.6 mm,5μm);流动相:0.1%磷酸水(A)-甲醇(B),梯度洗脱;流速0.8 mL·min-1;柱温为30℃;检测波长280 nm;进样量为10μL。对不同产地紫锥菊中酚酸含量进行聚类分析。结果 HPLC法可用于准确测定紫锥菊中酚酸类成分的含量,7种酚酸类成分在该色谱条件下有良好的分离度,在相应的线性范围内呈现良好的线性关系,平均回收率均在98.41%~101.9%,RSD<3.0%。结论建立的测定方法简便、准确,结合层次聚类分析可用于紫锥菊及其提取物的质量控制。Objective To establish a method for the determination of seven phenolic acids in Echinacea purpurea extracts from different producing areas by high performance liquid chromatography(HPLC),and to classify them by hierarchical clustering analysis(HCA),for the aim of providing basis for quality consistency and rational application.Methods The analysis was performed on an Inertsil C 18 column(250 mm×4.6 mm,5μm),mobile phase of 0.1%phosphoric acid water(A)-methanol(B)in a linear gradient mode.The flow rate was 0.8 mL·min-1.The detector wavelength was 280 nm and the column temperature was 30℃.The injection volume was 10μL.The content of phenolic acids in Echinacea purpurea from different areas was analyzed by cluster analysis.Results The content of phenolic acids in Echinacea purpurea could be accurately determined by HPLC.Seven phenolic acids had good separation resolution under this chromatographic condition,and showed good linear relationship in the corresponding linear range.The average recovery was in the range of from 98.41%to 101.9%,RSD<3.0%.Conclusion The established method is simple and accurate,which can be used for the quality control of Echinacea purpurea and its extracts by combining with hierarchical cluster analysis.

关 键 词：紫锥菊 酚酸类成分 色谱法 高效液相 含量测定 聚类分析 

分 类 号：R285[医药卫生—中药学] R927.2[医药卫生—中医学]